ACE UHPLC and HPLC Columns
Cortisol in Urine by LC-MS/MS
Conditions Analytes
Column: ACE Excel 2 C18
Dimensions: 100 x 2.1 mm
Part Number: EXL -101-1002U
Mobile Phase: 4 mM ammonium acetate in
H2O/0.2% (v/v) formic acid
in MeOH (71.5:28.5 v/v)
Flow Rate: 0.7 mL/min
Injection: 50 μL
Temperature: 50 °C
Detection: Applied Biosystems 5000 MS/MS
APCI in positive ion mode
Sample: BioRad Liquichek Urine Quality
Control standard (16 nmol/L cortisol)
Cortisol Cortisol-d4 (IS)
2.8e4
2.4e4
2.0e4
1.6e4
1.2e4
8000.0
8.0e4
7.0e4
6.0e4
5.0e4
4.0e4
3.0e4
1.0e4
0.0 0.0
0 1.0 0 1.0
Time - Minutes Time - Minutes
Reproduced with permission of Department of Pathology and Laboratory
Medicine, Heartlands Hospital, Birmingham, UK
2500
-50.00
2200
1000
-50.00
62
Application #AN2680
1. Cortisol
(m/z 363.5 121.3)
2. Cortisol-d4 (IS)
(m/z 367.3 331.3)
1
1 1
2
2 2
Cyclosporin Mixture
1
2
3
4
5
6
7 8 9
0 20 40 60
100
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Application #AN3270
Conditions Analytes
1. Isocyclosporin A
2. Cyclosporin C
3. Cyclosporin B
4. Cyclosporin L
5. Cyclosporin U
6. Cyclosporin A
7. Dihydrocyclosporin A
8. Cyclosporin G
9. Cyclosporin D
10. Cyclosporin E
Column: ACE 5 C18
Dimensions: 250 x 3.0 mm
Part Number: ACE-121-2503
Mobile Phase: H2O/MeCN/MTBE/H3PO4
(46:51:3:0.1 v/v/v/v)
Flow Rate: 0.8 mL/min
Temperature: 80 °C
Detection: UV, 210 nm
Time - Minutes
Reproduced with permission of Camurus AB, Lund, Sweden
10
0
Cyclodextrin-Encapsulated Flavour Compounds in Beer
Application #AN2300
Conditions Analytes
1. 2-Methylphenol
2. 2-Bromophenol
3. 2-Methoxy-4-vinylphenol
4. 4-Ethylphenol
5. 2,4-Dichlorophenol
6. 2,4,6-Trimethylphenol
7. 2-Isopropylphenol
Column: ACE 3 C18
Dimensions: 150 x 4.0 mm
Part Number: ACE-111-1504
Mobile Phase: 0.1% phosphoric acid in
MeOH/H2O (53:47 v/v)
Flow Rate: 0.5 mL/min
Injection: 20 μL
Temperature: 35 °C
Detection: UV, 225 nm and 261 nm
Reproduced with permission of FlavorActiV Ltd, UK
2
2
3
4
4
5
5
6
6
7
7
1
1
Time - Minutes
225 nm
261 nm
0.0 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0 11.3 12.5 13.8 15.0 16.3 17.5 18.8
1000
4000.0
2.0e4
2.0 3.0 4.0 5.0 6.0 2.0 3.0 4.0 5.0 6.0
mAU
3