ACE UHPLC and HPLC Columns
2
2
4
4
5
5
3
6
2 4 6 8 10 min
1
1
Time - Minutes
1
3
2
4
5
6
2
3
1 2 3 4 9min
Time - Minutes
2.4e4
2.4e4
2.2e4
2.2e4
2.0e4
2.0e4
1.8e4
1.8e4
1.6e4
1.6e4
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Cold Medicine Analytes (I) and (II)
Application #AN1940 and #AN2410
App #AN2410 App #AN1940
Conditions Analytes
1. Ascorbic acid
2. Paracetamol
3. Caffeine
4. Aspirin
5. Ethenzamide
6. Salicylic acid
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: (I) 20 mM phosphoric acid in
MeOH/H2O (25:75 v/v)
(II) 0.1% formic acid in
MeOH/H2O (55:45 v/v)
Flow Rate: (I) 1.5 mL/min (II) 1.0 mL/min
Injection: 5 μL
Temperature: (I) 40 °C (II) 25 °C
Detection: (I) UV, 280 nm (II) UV, 275 nm
Ascorbic acid Paracetamol Caffeine
Aspirin Ethenzamide Salicylic acid
1
6
5
4
1
4
3
6
1 2 3 4 5 6 7 8 minutes
1.4e4
1.4e4
1.2e4
1.0e4
1.0e4
8000
8000
6000
6000
4000
4000
2000
0
9 10 11 12
1.2e4
Corticosteroids by LC-MS/MS
Application #AN1030
Conditions Analytes
1. Triamcinolone
2. Prednisolone
3. Fluoroprednisolone
4. Methylprednisolone
5. Betamethasone
6. Dexamethasone
7. Flumethasone
Column: ACE 3 C18-PFP
Dimensions: 150 x 2.1 mm
Part Number: ACE-1110-1502
Mobile Phase: A: 0.1% formic acid in H2O
B: MeCN/0.1% formic acid in
H2O (90:10 v/v)
Gradient: Time (mins) %B
0 30
14 50
17 95
20 30
Flow Rate: 0.3 mL/min
Injection: 25 μL
Temperature: 15 °C
Detection: Turbospray, MRM
Reproduced with permission of Laboratorio Salud Publica de Burgos, Spain
1
2
3
4
5
6
7
(I)
(II)
1 10 Time - Minutes
2000
1 4 5 6 7 8 9
Intensity, cps