ACE UHPLC and HPLC Columns
ACE Advanced Method Development Kit
• Contains ACE C18, ACE C18-AR and ACE C18-PFP phases
• Ideal starting point for routine method development
• Available from microbore (0.5 mm id) through to analytical (4.6 mm id) dimensions
• Particularly recommended for compounds containing aromatic rings
Phase
Functional
Group
Endcapped
Particle Size
(μm)
Pore Size
(Å)
Surface
Area (m2/g)
Carbon
Load (%)
Recommended
pH Range
100%
Aqueous
Compatible
USP
Listing
ACE C18 Octadecyl (C18) Yes 1.7, 2, 3, 5, 10 100 300 15.5 2.0-8.0a No L1
ACE C18-AR
C18 with integral
Phenyl
Yes 1.7, 2, 3, 5, 10 100 300 15.5 2.0-8.0a Yes L1
ACE C18-PFP
C18 with integral
PFP
Yes 1.7, 2, 3, 5, 10 100 300 14.3 2.0-8.0a Yes L1
a For optimum column lifetime, a pH range of 2-8 is recommended. To increase column lifetime at higher pH, organic buffers, low buffer concentrations, high % organic solvent and low
temperatures must be considered. Further information is contained within “A Guide to HPLC and LC/MS Buffer Selection” by John Dolan – please contact your distributor to request your
FREE copy or visit www.ace-hplc.com
ACE C18 ACE C18-AR ACE C18-PFP
ACE C18 remains the “go-to”
column of choice for HPLC and
UHPLC separations. With an excellent
reputation for performance,
reproducibility and lifetime, ACE C18
provides a rugged, reproducible starting
point for method development.
Recommended Applications
• Analytes differing in hydrophobicity
• Polar, moderately polar and non-polar
analytes
• Uncharged acids and bases
• Ionized acids or bases using ion-pairing
• Ideal starting point for method
development
ACE C18-AR combines the excellent
performance and advantages of the ACE
C18 phase with the added selectivity of an
integral phenyl group.
Recommended Applications
• Analytes with π-bonding and conjugated
systems
• Analytes with electron delocalization and
electron withdrawing groups, such as
halogens, nitro groups, ketones, esters
and acids
• Analytes with different dipole moments
• Analytes differing in hydrophobicity
• Stereoisomers, steroids, substituted
aromatics and sulphur containing
compounds
• Fully wettable - 100% aqueous buffer
compatible
• Applications where C18 does not provide
adequate separation
• Applications where conventional phenyl
phases provide insufficient retention, poor
stability, or significant bleed.
ACE C18-PFP brings together the
stability, reproducibility and low bleed
of the ACE C18 phase with the additional
selectivity of an integral
pentafluorophenyl (PFP) group.
Recommended Applications
• Analytes with π-bonding
• Analytes with electron donating groups,
such as phenols, aromatic ethers and amines
• Analytes with proton donor groups
• Analytes with different dipole moments
• Analytes differing in hydrophobicity
• Stuctural isomers, steroids, substituted aromatics
and taxanes
• Fully wettable - 100% aqueous buffer compatible
• Applications where C18 does not provide adequate
separation
• Applications where conventional PFP
phases provide insufficient retention,
poor stability or significant bleed.
Additional Information
Product bulletins containing further details on the ACE C18,
C18-AR and C18-PFP columns contained within the
Advanced ACE Method Development Kit are available to
download at www.ace-hplc.com Alternatively,
please contact our technical support team via
info@ace-hplc.com or contact your local distributor.
Learn More: www.ace-hplc.com ACE
ACE
HPLC and UHPLC Columns
Product Catalogue
Explore
Selectivity
2 and 3 column kits
available for the
same price as a
single column
6
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com