ACE UHPLC and HPLC Columns
Analytes
1. Cefquinome
(m/z 529.2)
2. Excipient
1 2
Ciprofibrate
Furosemide (IS)
Time - Minutes Time - Minutes
TIC of 3 MRMs of 0.02 ppb chloramphenicol spiked in milk matrix
MRM chromatograms of 0.05 ppb chloramphenicol in milk
6
5
4
3
2
10.0
7.5
5.0
2.5
0.0
6
5
4
3
2
6
5
4
3
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Cefquinome by LC-MS
Conditions
Column: ACE 5 C18
Dimensions: 150 x 2.1 mm
Part Number: ACE-121-1502
Mobile Phase: A: 2 mM formic acid in H2O
B: 2 mM formic acid in MeCN
Gradient: Time (mins) %B
0 5
1 5
10 95
30 95
Flow Rate: 0.2 mL/min
Temperature: 25 °C
Detection: ESI-MS (+)
Time - Minutes
Application #AN3130
1
Cefquinome
2
0 5 10 15 20 25 30
Reproduced with permission of Department of Soil Science,
University of Reading, UK
Ciprofibrate from Human Plasma
by LC-MS/MS
Conditions Analytes
1. Ciprofibrate
(m/z 287.0 85.0)
2. Furosemide (IS)
(m/z 328.9 204.9)
Column: ACE 5 C18
Dimensions: 50 x 4.6 mm
Part Number: ACE-121-0546
Mobile Phase: 0.001% ammonia in MeOH/
MeCN/H2O (70:20:10 v/v/v)
Flow Rate: 1 mL/min
Injection: 20 μL
Temperature: Ambient
Detection: API 3200 triple quad MS
ESI in negative ion mode
Ion source temperature: 550 °C
Ion spray voltage: 4500 V
Application #AN2670
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 mins 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6
Reproduced with permission of Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar, Gujarat, India
m/z 320.9 → 152
Quan.fica.on
m/z 320.9 → 257
Qualifica.on
m/z 320.9 → 194
Qualifica.on
Chloramphenicol in Milk by LC-MS/MS
Conditions Analyte
Column: ACE 3 C18 1. Chloramphenicol
Dimensions: 50 x 2.1 mm
Part Number: ACE-111-0502
Mobile Phase: A: H2O
B: MeOH
Gradient: Time (mins) %B
0.00 10
0.05 10
2.50 95
3.00 95
3.10 10
4.50 10
Flow Rate: 0.5 mL/min
Injection: 10 μL
Detection: Bruker EVOQ Elite triple quad MS
VIP heated-ESI temperature: 400 °C
Cone gas temperature: 350 °C
Spray voltage: -4500 V
Time - Minutes
Reproduced with permission of Bruker UK Ltd
CAP (0.02 ppb in milk matrix)
Milk Matrix Blank
Application #AN2030
2.0
1
1
1
0
2 3 4
1
2
2.1 2.2 2.3 2.4