ACE UHPLC and HPLC Columns
Catechols Mixture Separations (I) and (II)
Conditions Analytes
Column: (I) ACE Excel 3 CN-ES (II) ACE Excel 3 C18-Amide
Dimensions: 150 x 4.6 mm
Part Number: (I) EXL -1113-1546U (II) EXL -1112-1546U
Mobile Phase: (I) 20 mM H3PO4 in MeCN/H2O (25:75 v/v)
(II) 20 mM H3PO4 in MeCN/H2O (10:90 v/v)
Flow Rate: 1.5 mL/min
Injection: 5 μL
Temperature: 30 °C
Detection: UV, 270 nm
1 2
1
3
2
4
5 2-Methylresorcinol 4-Methylcatechol
5
6
1. Resorcinol
2. Catechol
3. 2-Methylresorcinol
4. 4-Methylcatechol
5. 3-Methylcatechol
6. 4-Nitrocatechol
2.5 5
7.5 10 12.5 15 17.5 min
2.5
2.5
5
7.5
7.5
10
10
12.5
12.5
15
15
17.5
17.5
0
0 2.5 5 7.5 10 12.5 15 17.5
800
600
400
200
Response (mAU)
56
1
2
3
4
5
6
UV Detection Amperometric Detection
0 2 6 10 14
25
20
15
10
5
4 6 8 10 12
Current (μA)
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com
Application #AN1430 and #AN1440
App #AN1440 App #AN1430
Resorcinol Catechol
3-Methylcatechol
4-Nitrocatechol
3 4
6
1
3
4
5
6
Time - Minutes
(I)
(II)
Cathinone Psychoactive Substances by LC-UV and LC-Amperometry
Conditions Analytes
1. Caffeine
2. 4-Methylmethcathinone
(4-MMC, mephedrone)
3. 4-Methylethcathinone (4-MEC)
Column: ACE 3 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-111-1546
Mobile Phase: 10 mM ammonium acetate-100
mM KCl pH 4.3/MeOH (70:30 v/v)
Flow Rate: 0.8 mL/min
Injection: 10 μL
Temperature: 22 °C
Detection: UV, 264 nm
Amperometric Potential +1.4 V
Application #AN3500
1
2
3
1
3
2
1
2
3
1
2 3
Caffeine
4-Methylmethcathinone
(4-MMC, mephedrone)
4-Methylethcathinone (4-MEC)
Time - Minutes Time - Minutes
Zuway K, Smith J, Foster C, Kapur N, Banks C, Sutcliffe O, (2015) Detection and quantification of new psychoactive substances (NPSs) within the evolved
‘legal high’ product, NRG-2, using high performance liquid chromatography-amperometric detection (HPLC-AD). Analyst 140, 6283. doi:10.1039/c5an01106j