ACE UHPLC and HPLC Columns
Catecholamines and Metanephrines Separation (Isocratic)
Conditions Analytes
1. Norepinephrine
2. Epinephrine
3. Normetanephrine
4. Dopamine
5. Metanephrine
5
5
Noradrenaline Adrenaline
Catecholamines from Urine
1
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 55
Column: ACE 5 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: ACE-1210-1546
Mobile Phase: 20 mM ammonium formate pH 3.0 in H2O
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 25 °C
Detection: UV, 260 nm
Application #AN1490
Norepinephrine Epinephrine
Normetanephrine Dopamine
Metanephrine
1
2
3
4
1
2
3
4
2 4 6 8 10 12 14 min
Time - Minutes
Catecholamines from Plasma
Conditions Conditions
Time - Minutes
Application #AN3210
Analytes Analytes
1. Noradrenaline (Norepinephrine)
2. 3,4-Dihydroxynorephedrine (I.S.)
3. Adrenaline (Epinephrine)
1. Noradrenaline (Norepinephrine)
2. 3,4-Dihydroxynorephedrine (I.S.)
3. Adrenaline (Epinephrine)
4. Dopamine
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 50 mM sodium acetate
pH 7.0/MeCN/MeOH (50:35:15 v/v/v)
Flow Rate: 0.9 mL/min
Temperature: Ambient
Detection: Fluorescence – lEx 350 nm, lEm 480 nm
Sample: Ion pair extraction using diphenyl-borate-ethanolamine.
Derivatisation using diphenyl-ethylenediamine as
fluorescent probe
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 50 mM sodium acetate
pH 7.0/MeCN/MeOH
(50:35:15 v/v/v)
Flow Rate: 0.9 mL/min
Temperature: Ambient
Detection: Fluorescence – lEx 350 nm, lEm 480 nm
Sample: Ion pair extraction using diphenyl-borate-ethanolamine.
Derivatisation using diphenyl-ethylenediamine as
fluorescent probe
Reproduced with permission of MAS University Hospital, Malmo, Sweden
Noradrenaline
Time - Minutes
Application #AN3200
0 5 10 15 0 5 10 15
Reproduced with permission of MAS University Hospital, Malmo, Sweden
1
2
2
3
3
4
Dopamine
3,4-Dihydroxynorephedrine (I.S.)
3,4-Dihydroxynorephedrine (I.S.) Adrenaline