ACE UHPLC and HPLC Columns
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Cannabinoids (Synthetic) by LC-MS/MS
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Conditions
Application #AN2540
Column: ACE Excel 3 C18-AR
Dimensions: 100 x 3.0 mm
Part Number: EXL -119-1003U
Mobile Phase: A: 15 mM ammonium formate pH 4.0 in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0.00 40
3.74 90
8.00 90
8.50 40
Flow Rate: 0.5 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: Shimadzu LCMS 8040 MS
Positive ion ESI
Analytes
1. JWH-018 N-5-OH-pentyl-d5
(m/z 362.90 155.05; 127.00; 128.05)
2. JWH-250 N-5-OH-pentyl
(m/z 352.20 121.15; 91.10; 186.05)
3. JWH-073 N-4-OH-butyl
(m/z 344.20 155.00; 127.10; 54.95)
4. JWH-018 N-pentanoic
(m/z 372.20 155.05; 127.10)
5. JWH-018 N-5-OH-pentyl
(m/z 357.80 155.05; 127.05)
6. AM2201 N-4-OH-pentyl
(m/z 376.40 155.00; 127.00; 144.00)
7. AM2201 5/6-OH-indole
(m/z 375.90 155.05; 127.05; 248.10)
8. JWH-081 N-5-OH-pentyl
(m/z 388.20 185.05; 157.05; 114.15)
9. MAM2201 N-4-OH-pentyl
(m/z 389.60 169.00; 141.05; 115.15)
10. AB-CHMINACA
(m/z 356.70 241.05; 312.20; 340.15)
11. UR-144 N-pentanoic
(m/z 341.60 125.10; 55.05; 57.10)
12. JWH-019 N-6-OH-hexyl
(m/z 371.80 155.05; 127.00; 144.00)
13. JWH-122 N-5-OH-pentyl
(m/z 372.20 169.05; 141.05; 115.15)
14. AKB48 N-pentanoic
(m/z 395.60 135.00; 93.10; 79.05)
15. JWH-018 5-OH-indole
(m/z 358.20 155.00; 127.05; 230.05)
16. AKB48 N-5-OH-pentyl
(m/z 381.60 135.10; 93.10; 79.05)
17. JWH-210 5-OH-indole
(m/z 386.10 183.05; 153.10; 155.05)
18. PB-22
(m/z 358.80 214.05; 144.05; 116.00)
19. JWH-073
(m/z 328.20 127.10; 155.05; 200.10)
20. EAM2201
(m/z 387.70 183.10; 232.10; 155.10)
21. JWH-122 N-4-pentenyl
(m/z 353.70 169.05; 141.10; 115.10)
22. JWH-018
(m/z 341.70 155.00; 127.05; 214.10)
23. JWH-081
(m/z 372.10 185.05; 157.15; 127.10)
24. AKB48F
(m/z 384.30 135.15; 107.10; 93.10)
25. THJ-018
(m/z 342.60 215.10; 145.05; 90.00)
26. JWH-122
(m/z 356.30 169.05; 141.10; 115.15)
27. JWH-210
(m/z 370.10 183.10; 155.10; 153.10)
3.00
6000000
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0
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3.25 3.50 3.75 4.00 4.25 4.50 4.75 5.00 5.25
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Reproduced with permission of ISZKI (Institute of Toxicology), Hungary