ACE UHPLC and HPLC Columns
Caffeine and Metabolites
5
5 10 15 20 25 30 min
Caffeoylquinic and Dicaffeoylquinic Acids
Conditions
50
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Application #AN3520
Analytes
1. 3-Caffeoylquinic acid (chlorogenic acid)
2. 5-Caffeoylquinic acid (neochlorogenic acid)
3. 4-Caffeoylquinic acid (cryptochlorogenic acid)
4. Feruloylquinic acid
5 3,4-Dicaffeoylquinic acid (isochlorogenic acid B)
6. 3,5-Dicaffeoylquinic acid (isochlorogenic acid A)
7. 4,5-Dicaffeoylquinic acid (isochlorogenic acid C)
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 4.6 mm
Part Number: CORE-25A-1046U
Mobile Phase: A: 0.2% phosphoric acid in H2O
B: MeCN
Gradient: Time (mins) %B
0 5
1 5
9 18
14 28
15 70
Flow Rate: 0.8 mL/min
Temperature: 35 °C
Detection: UV-Vis, 327 nm
1
2
3
4 5
6 7
Conditions
Application #AN2010
Analytes
1. Xanthine
2. Theobromine
3. Paraxanthine
4. Theophylline
5. Caffeine
Column: ACE Excel 5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: EXL -1211-1546U
Mobile Phase: A: 20 mM ammonium
acetate pH 7.0 in H2O
B: 20 mM ammonium acetate
pH 7.0 in MeCN/H2O (90:10 v/v)
Gradient: Time (mins) %B
0 2
45 15
48 15
49 2
59 2
Flow Rate: 1 mL/min
Injection: 1 μL
Temperature: 60 °C
Detection: UV, 273 nm
Reproduced with permission of Dongling Health Food Co. Ltd, Zhejiang, China
Time - Minutes
Time - Minutes
1
2
3
4
5
0 5 10 15 20 25 30
Xanthine
Paraxanthine
Caffeine
Theobromine
Theophylline
5-Caffeoylquinic acid (neochlorogenic acid)
3,5-Dicaffeoylquinic acid (isochlorogenic acid A)