ACE UHPLC and HPLC Columns
Analytes
1. Pindolol
2. Metoprolol
3. Oxprenolol
4. Propranolol
Metoprolol
Oxprenolol
Propranolol
250
200
150
100
50
2
3
Benzo(a)pyrene-7,8-quinone Derived
Deoxynucleotide DNA Adducts
Conditions
4
Application #AN3170
BPQ-dG#1 BPQ-dG#2
Analytes
1. BPQ-dG#1
2. BPQ-dG#2
3. BPQ-dG#3
4. BPQ-dG#4
Column: ACE 3 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-111-1546
Mobile Phase: A: 0.1% formic
acid in H2O
B: MeCN
Gradient: Time (mins) %B
0 25
8 55
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 35 °C
Detection: UV, 285 nm
BPQ-dG#3 BPQ-dG#4
3 4
2
1
5 10 15 20 25 30
Time - Minutes
Reproduced with permission of Institute of Cancer Research, Sutton, Surrey, UK
b-Blockers by LC-MS/MS
Application #AN2620
Conditions
Column: ACE Excel 2 C18
Dimensions: 50 x 2.1 mm
Part Number: EXL -101-0502U
Mobile Phase: A: 2 mM ammonium acetate
+ 0.1% formic acid in H2O
B: 2 mM ammonium acetate
+ 0.1% formic acid in MeOH
Gradient: Time (mins) %B
0.0 10
3.0 50
3.1 10
Flow Rate: 0.4 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: MS/MS ESI in positive ion mode
Sample: 2.5 pg/μL
Analytes
1. Sotalol
(m/z 272.9 212.8)
2. Atenolol
(m/z 267.0 189.8)
3. Pindolol
(m/z 248.9 115.8)
4. Nadolol diastereomers
(m/z 310.0 253.9)
5. Metoprolol
(m/z 268.0 115.8)
6. Labetalol
(m/z 329.1 161.8)
7. Propranolol
(m/z 260.0 115.7)
8. Alprenolol
(m/z 250.0 115.8)
Sotalol Atenolol
Pindolol Nadolol diastereomers
Metoprolol Labetalol
Propranolol Alprenolol
Beta Blockers mix8 2.5 pg/uL (500pg recon 200uL) 90:10 H2O:MeOH 0.1%FA: ACE EXCEL 2 C18 50 x 2.1:
BB_181114_004 6: MRM of 2 Channels ES+
Time
2
0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80
100
%
0
260 > 115.7 (PROPANOLOL)
1.03e6
3.42
1 2 3 4
1
1
2
4
4
5
6
7
8
3
3
100
Time - Minutes
Reproduced with permission of Biotage GB Ltd, UK
Time - Minutes
b-Blockers
Conditions
Application #AN3160
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: 50 mM 1-methylpiperidine
pH 11/MeOH (35:65 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 215 nm
Pindolol
1
0 2
0
4 6 8 10 12 14 16
Response - MilliVolts
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