ACE UHPLC and HPLC Columns
b-Blockers at High pH
Conditions
Application #AN1420
Column: ACE Excel 5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: EXL -1211-1546U
Mobile Phase: A: 0.1% ammonia in H2O
B: 0.1% ammonia in MeCN
Gradient: Time (mins) %B
0.0 30
5.0 90
7.0 90
7.5 30
22.5 30
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 35 °C
Detection: UV, 230 and 254 nm
Analytes
1. Atenolol
2. Acebutalol
3. Pindolol
4. Metoprolol
5. Oxprenolol
6. Carvedilol
7. Propranolol
Atenolol Acebutalol
Pindolol
Metoprolol
Oxprenolol Carvedilol
Propranolol
1
2
3
4
5
6
7
1
2
3
4
5
6
7
Time - Minutes
3 min
mAU
mAU
160
160
140
140
120
120
100
100
80
80
60
60
40
40
20
0
b-Blockers at High pH – Fast Analysis
Conditions
Application #AN2160
Analytes
Column: ACE Excel 1.7 SuperC18
Dimensions: 50 x 3.0 mm
Part Number: EXL -1711-0503U
Mobile Phase: A: 0.1% ammonia in H2O
B: 0.1% ammonia in MeCN
Gradient: Time (mins) %B
0.0 30
4.3 55
5.0 55
6.0 30
9.0 30
Flow Rate: 1 mL/min
Injection: 0.7 μL
Temperature: 20 °C
Detection: UV, 230 nm
1. Atenolol
2. Acebutalol
3. Pindolol
4. Metoprolol
5. Oxprenolol
6. Carvedilol
7. Propranolol
1
1
2
2
3
3
4
4
5
6
7
Time - Minutes
20
0
44
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