ACE UHPLC and HPLC Columns
Artificial Food Colours
Conditions
Application #AN2960
Analytes
1. Tartrazine
2. Amaranth
3. Sunset Yellow
4. Ponceau 4R
Column: ACE 3 C18
Dimensions: 100 x 4.6 mm
Part Number: ACE-111-1046
Mobile Phase: A: 3.1 mM TBAB1 and 5 mM
KH2PO4 in H2O
B: 5 mM KH2PO4 in MeOH
Gradient: Time (mins) %B
0 45
12 60
25 45
Flow Rate: 0.8 mL/min
Injection: 10 μL
Temperature: Ambient
Detection: UV-VIS, 480 nm
1 Tetrabutylammonium bromide
1 2
3
4
0 2 4 6 8 10 12 14 16 18 20 22 24
18
16
14
12
10
8
6
4
2
0
Response - MilliVolts
Time - Minutes
Reproduced with permission of Lincolne Sutton & Wood, Norwich, UK
Artemisinin
Conditions
Application #AN3140
Analyte
Column: ACE 5 C18 1. Artemisinin
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: A: H2O
B: MeOH
Gradient: Time (mins) %B
0 50
25 100
35 100
Flow Rate: 1.0 mL/min
Injection: 20 μL
Temperature: 20 °C
Detection: ELSD
1
0 5 10 15 20 25 30 35
30
28
26
24
22
20
18
16
14
12
10
8
6
4
2
0
Response - MilliVolts
Time - Minutes
Reproduced with permission of Neem Biotech, Cardiff, UK
Artificial Sweeteners Global Method
Conditions
Application #AN1980
Analytes
1. Acesulfame K
2. Cyclamate
3. Saccharin
4. Sucralose
5. Aspartame
6. Neotame
7. Alitame
8. Neohesperidin
dihydrochalcone
9. Dulcin
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: A: H2O
B: MeCN
C: 0.1% TFA
Gradient: Time (mins) %A %B %C
0 88 2 10
25 50 40 10
30 30 60 10
35 88 2 10
Flow Rate: 1 mL/min
Injection: 50 μL
Temperature: 30 °C
Detection: Corona CAD
1
2
3
4
5
6
7
8
9
Time - Minutes
Response
Reproduced with permission of Durham County Council Scientific Services, UK
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