ACE UHPLC and HPLC Columns
Antimycins from Marine Sponge Streptomyces sp. by LC-HRMS
Conditions
Column: ACE 5 C18
Dimensions: 75 x 3.0 mm
Part Number: ACE-121-7503
Mobile Phase: A: 0.1% (v/v) formic acid in H2O
B: 0.1% (v/v) formic acid in MeCN
Gradient: Time (mins) %B
0 10
30 100
35 100
Flow Rate: 0.3 mL/min
Injection: 10 μL
Detection: Thermo Exactive Orbitrap
High resolution (15,000)
ESI in positive ion mode
Spray Voltage: 4.5 kV
Capillary Temperature: 268 °C
Capillary Voltage: 30 V
Sample: Antifungal fraction from Streptomycin sp.
SM8 extract from Haliclona simulans marine sponge
Application #AN4380
Antimycin A
Antimycin R1 R2
A1a C=OCH(CH3)CH2CH3 (CH2)5CH3
A1b C=OCH2CH(CH3)2 (CH2)5CH3
A2a C=OCH(CH3)2 (CH2)5CH3
A2b C=OCH2CH2CH3 (CH2)5CH3
A3a C=OCH(CH3)CH2CH3 (CH2)3CH3
A3b C=OCH2CH(CH3)2 (CH2)3CH3
A4a C=OCH(CH3)2 (CH2)3CH3
A4b C=OCH2CH2CH3 (CH2)3CH3
A5 C=OCH2CH(CH3)2 CH2CH3
A6 C=OCH2CH2CH3 CH2CH3
A1
A2
A4
A6
A5
A3
Time - Minutes
Base peak intensity
––––– Antimycin A standard –––––– Extract from SM8 wild type grown on oatmeal culture media
Viegelmann C, Margassery LM, Kennedy J, Zhang T, O’Brien C, O’Gara F, Morrissey JP, Dobson ADW, Edrada-Ebel R. Metabolomic profiling and genomic study
of a marine sponge-associated Streptomyces sp. Marine Drugs 12, 3323-3351 (2014). doi:10.3390/md12063323
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