Antihistamines and Expectorants – Mobile Phase
Effects
Conditions
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: (a) MeOH/50 mM KH2PO4
(pH 3.0) (45:55 v/v)
(b) MeCN/50 mM KH2PO4
(pH 3.0) (28:72 v/v)
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detection: UV, 220 nm
Analytes
1. Maleic acid
2. Salicylamide
3. Guaifenesin
4. Guaiacol
5. Chlorpheniramine maleate
6. Dextromethorphan
700
600
500
400
300
200
100
900
800
700
600
500
400
300
200
100
Time - Minutes
Response - MilliVolts Response - MilliVolts
(b) Acetonitrile based mobile phase
1
2
3
4
5 6
1
3 2
4
5 6
0
(a) Methanol based mobile phase
0
0
1
1
2
2
3
3
5
5
4
4
7
7
6
6
8
8
10
10
9
9
12
12
11
11
14
14
15
15
13
13
0
Application #AN3960
Anti-Ulcer Drugs in Basic Mobile Phase Conditions
1
2
Conditions
Time - Minutes
Application #AN1950
Analytes
1. Omeprazole
2. Lansoprazole
Column: ACE 5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: EXL -1211-1546U
Mobile Phase: A: 0.1% ammonia in H2O
B: 0.1% ammonia in MeCN/H2O
(90:10 v/v)
Gradient: Time (mins) %B
0 10
5 90
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 30 °C
Detection: UV, 280 nm
Omeprazole
Lansoprazole
1 2 3 4 5
ACE UHPLC and HPLC Columns
Antihistamines – Fast Analysis
Conditions
Time - Minutes
Application #AN4290
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 50 x 3.0 mm
Part Number: CORE-25A-0503U
Mobile Phase: 30 mM KH2PO4 pH 2.7 in
H2O/MeOH (60:40 v/v)
Flow Rate: 0.85 mL/min
Injection: 0.9 μL
Temperature: 30 °C
Detection: UV, 214 nm
Analytes
1. Maleic acid
2. Norephedrine
3. Doxylamine
4. Salicylamide
5. Guaifenesin
6. Guaiacol
7. Chlorpheniramine
8. Triprolidine
0.25 0.5 0.75 1 1.25 1.5 1.75 2 2.25 min
mAU
200
150
100
50
0
3
1
2
4
5
6
7 8
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