ACE UHPLC and HPLC Columns
Andrographis Paniculata Fingerprint Profile by RRLC-TOF-MS
2
5
1
Analytes
1. 14-Deoxyandrographiside
2. Andrographolide
3. Neoandrographolide
4. 14-Deoxyandrographolide
5. Dehydroandrographolide
2.5 5.0 7.5 10.0
Conditions
100
%
0
3
4
Time - Minutes
Application #AN3770
14-Deoxyandrographiside
(*Tentative assignment)
Andrographolide
Neoandrographolide 14-Deoxyandrographolide
(*Tentative assignment)
Dehydroandrographolide
Song Y-X, Liu S-P, Jin Z, Qin J-F, Jiang Z-Y (2013) Qualitative and quantitative analysis of
Andrographis Paniculata by rapid resolution liquid chromatography/time-of-flight mass spectrometry. Molecules 189, 12192-12207 doi:10.3390/molecules181012192
Angiotensin II Receptor Antagonists by LC-UV
Application #AN3460
Analytes
1. Valsartan
2. Losartan
3. Irbesartan
4. Telmisartan
Conditions
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: 0.025 M KH2PO4 pH 6.0/MeCN
(65:35 v/v)
Flow Rate: 1.5 mL/min
Temperature: 40 °C
Detection: UV, 220 nm
mAU
800
600
400
200
0
Valsartan Losartan
1
2
3
4
1
2
3
4
0 5 10
Time - Minutes
Irbesartan
Elshanawane AA, Abdelaziz LM, Hafez HM (2012) Stability Indicating
HPLC Method for Simultaneous Determination of Several Angiotensin-II
Receptor Antagonists in Their Dosage Forms. Pharmaceut Anal Acta 3:175.
doi:10.4172/2153-2435.1000175
Andrographis Paniculata -
used in Chinese medicine.
For further applications
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Column: ACE Excel 3 SuperC18
Dimensions: 100 x 2.1 mm
Part Number: EXL -1111-1002U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0.0 30
2.0 40
8.0 75
9.0 100
9.5 30
15.5 30
Flow Rate: 0.2 mL/min
Injection: 2 μL
Temperature: 35 °C
Detection: Waters Premier Q-TOF-MS
ESI in positive ion mode
Scan Range m/z 100-1000 Da
3
4
5
Telmisartan
34
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