1
1
2
2
m/z 329.0 106.8
m/z 329.0 94.8
m/z 271.1 146.8
m/z 271.1 120.8
ACE UHPLC and HPLC Columns
Anabolic Steroids from Horse Urine by LC-MS/MS
Conditions
Application #AN2360
Analytes
1. Stanozolol
2. Ethylestrenol
Column: ACE Excel 2 C18
Dimensions: 50 x 2.1 mm
Part Number: EXL -101-0502U
Mobile Phase: A: 2 mM ammonium acetate, 0.1%
formic acid in H2O
B: 2 mM ammonium acetate, 0.1%
formic acid in MeOH
Gradient: Time (mins) %B
0.00 75
0.25 75
1.50 90
1.51 100
3.50 100
3.51 75
4.00 75
Flow Rate: 0.4 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: Premier XE triple quad MS
MRM positive ion mode
Desolvation temperature: 450 °C
Ion Source temperature: 120 °C
Time - Minutes
Stanozolol Ethylestrenol
Reproduced with permission of Biotage GB, Ltd. For extraction conditions see Biotage Application Note AN843
Analgesic Rapid Separation
Conditions
2
3
Time - Minutes
Application #AN1370
Analytes
1. Paracetamol
2. Hydrochlorothiazide
3. Aspirin
4. Bendroflumethiazide
5. Ketoprofen
6. Flurbiprofen
7. Ibuprofen
Column: ACE Excel 2 SuperC18
Dimensions: 50 x 2.1 mm
Part Number: EXL -1011-0502U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0.0 5
1.0 100
1.5 100
1.6 5
3.1 5
Flow Rate: 1.2 mL/min
Injection: 0.5 μL
Temperature: 50 °C
Detection: UV, 214 nm
1
4 5
6
7
0.2 0.4 0.6 0.8 min
Paracetamol Hydrochlorothiazide
Aspirin Bendroflumethiazide
Ketoprofen Flurbiprofen
Ibuprofen
2
1
3
4 5
6
7
32
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