ACE UHPLC and HPLC Columns
5,6,8
12
5,6,8
9
9
10
10
12
Time - Minutes
100
Reproduced with permission of Biotage GB Ltd, UK
%
0
2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 5.2 5.4 5.6 5.8 6.0
Amphetamines from Drugs of Abuse Screen (#AN2190)
Conditions
Application #AN2350
Analytes
5. Amphetamine-d5
(m/z 141.0 123.9)
6. Amphetamine
(m/z 136.0 118.9)
8. MDA
(m/z 180.1 105.0)
9. MDMA
(m/z 194.1 163.0)
10. Methamphetamine
(m/z 150.0 90.9)
12. MDEA
(m/z 208.2 163.0)
Column: ACE Excel 1.7 C18
Dimensions: 100 x 2.1 mm
Part Number: EXL -171-1002U
Mobile Phase: A: 5 mM ammonium acetate in H2O
B: 5 mM ammonium acetate in MeOH
Gradient: Time (mins) %B
0.0 10
10.0 90
11.9 90
13.4 10
15.5 10
Flow Rate: 0.3 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: MS Quattro Premier XE triple quad
MRM, positive and negative ESI mode
Desolvation temperature: 450 °C
Ion source temperature: 150 °C
Collision gas pressure: 3.5 x10-3 mbar
Amphetamines in Urine by LC-MS/MS
Conditions
Application #AN1010
Analytes
1. Phenylpropanolamine
LOD (est) 4 ppb
(m/z 151.6 134.0)
2. (l)-Ephedrine
LOD (est) 2 ppb
(m/z 166.2 148.0)
3. (dl)-3,4-MDA
LOD (est) 30 ppb
(m/z 179.7 163.0)
4. (d)-Amphetamine
LOD (est) 4 ppb
(m/z 135.8 90.9)
5. (dl)-3,4-MDMA
LOD (est) 2 ppb
(m/z 193.7 163.0)
6. 4-Methylthioamphetamine
LOD (est) 10 ppb
(m/z 182.2 165.0)
7. (±)-MDEA
LOD (est) 1 ppb
(m/z 207.7 165.0)
Column: ACE Excel 3 SuperC18
Dimensions: 75 x 2.1 mm
Part Number: EXL -1111-7502U
Mobile Phase: A: 5 mM ammonium hydroxide
pH 10.8 in H2O
B: 5 mM ammonium hydroxide pH 10.8
in MeOH/H2O (90:10 v/v)
Gradient: Time (mins) %B
0 30
8 95
Flow Rate: 0.6 mL/min
Injection: 2 μL
Temperature: 60 °C
Detection: Varian 320 Triple Quadrupole MS
Electrospray voltage: +5 kV
Inlet capillary voltage: 30 V
CID with argon at 1.5 mTorr
Collision cell potential ranges
from 5 to 17 V
Drying gas (nitrogen) temperature: 325 °C
Nebulizing gas (nitrogen) pressure: 35 psi
Extended Dynamic Range
2
5
6
7
1
3
4
1 2 3 4 5 6 7 8
MMCCoouunntt
15.0
12.5
10.0
7.5
5.0
2.5
0.0
Phenylpropanolamine (dl)-3,4-MDMA
(dl)-3,4-MDA (±)-MDEA
(l)-Ephedrine (d)-Amphetamine
4-Methylthioamphetamine
1 2 3 4 5 6 7 8
15.0
12.5
10.0
7.5
5.0
2.5
0.0
1
2
3
4
5
6
7
Time - Minutes
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 31