ACE UHPLC and HPLC Columns
Amoxicillin Metabolites in Human Liver Microsomes
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Peak Analyte M+H+ Elemental
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Conditions
Column: ACE 5 C18-300
Dimensions: 150 x 4.6 mm
Part Number: ACE-221-1546
Mobile Phase: 0.1% (v/v) formic acid in H2O/MeCN (35:65 v/v)
Flow Rate: 0.4 mL/min
Injection: 15 μL
Temperature: 21 °C
Detection: Agilent 6410 triple quad MS
ESI in positive ion mode
Full scan MS and MS/MS data obtained
Sample: In vitro incubation of amoxicillin with human liver
microsomes in the presence of NADPH
Application #AN4400
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Time - Minutes
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Composition
Metabolic Reaction
1 M1 382 C16H20N3O6S Hydroxylation
2 M2 379 C17H19N2O7S Oxidative deamination
3 M3 382 C16H20N3O6S Oxidation of aliphatic chain
4 M4 380 C16H18N3O6S Oxidation of aliphatic chain
5 M5 396 C16H20N3O7S Oxidation of aliphatic chain
6 M6 322 C15H19N3O3S Decarboxylation
7 M7 542 C25H28N3O11S Glucuronidation
8 Amoxicillin 366 C16H20N3O5S
Relative Abundance
Amoxicillin
Szultka M, Krzeminski R, Jackowski M, Buszewski B. (2014) Identification of in vitro Metabolites of Amoxicillin in Human Liver Microsomes by LC-ESI/MS,
Chromatographia, 77 , 1027-1035. doi 10.1007/s10337-014-2648-2
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