Aminoglycosides in Eggs
Conditions Analytes
KNM (485 > 163)
ACE UHPLC and HPLC Columns
Reproduced with permission of Veterinary Drug Residues Department, Centro Nacional de Alimentación (CNA, AECOSAN), Madrid, Spain
Application #AN1920
Time - Minutes
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL -1010-1002U
Mobile Phase: A: 20 mM HFBA in H2O/MeCN (98:2 v/v)
B: 20 mM HFBA in MeCN/H2O (98:2 v/v)
Gradient: Time (mins) %B Curve
0.0 5.0 -
2.0 15.0 6
4.5 19.0 6
5.5 19.5 8
6.0 22.0 6
7.0 35.0 6
9.0 48.0 8
9.5 5.0 6
Flow Rate: 0.4 mL/min
Temperature: 40 °C
Detection: Positive ESI MRM (transitions as shown)
Sample: Extraction at low pH, clean up with WCX SPE cartridge
Egg sample spiked at 100 μg/kg (CCa)
GNT Gentamicin
TBM Tobramycin
KNM Kanamycin
DHS Dihydrostreptomycin
STR Streptomycin
HGB Higromycin-B
SPT Spectinomycin
GNT-C1 (478 > 322)
RT: 8.40
RT: 8.40
GNT-C2a
RT: 8.26
RT: 8.09
RT: 7.52
RT: 5.65
RT: 5.48
RT: 5.09
RT: 3.67
RT: 8.31
GNT-C2 (464 > 322) GNT-C2
GNT-C1a (450 > 322)
TBM (468 > 163)
DHS (584 > 263)
STR (582 > 263)
HGB (528 > 177)
SPT (351 > 333)
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