ACE UHPLC and HPLC Columns
Amino Acids in Peas (Pisum Sativum) by HPLC-HRAM-MS
Conditions
Column: ACE 3 AQ
Dimensions: 150 x 3.0 mm
Part Number: ACE-116-1503
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0 0
10 100
Flow Rate: 0.4 mL/min
Injection: 5 μL
Temperature: 30 °C
Detection: Exactive Orbitrap high resolution MS
ESI positive ion mode
Capillary temperature: 350 °C
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
Reproduced with permission of Fera Science Ltd, York, UK
Application #AN2660
Time - Minutes
Arg Ser
0 1 12
Time - Minutes
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
100
80
60
40
20
0
2 3 4 5 6 7 8 9 10 11
0 1
20
0
2 3 4 5 6 7 8 9 10 11 12
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
Relative Abundance
Relative Abundance
Asn
Val
Ala
Met
Tyr
Phe
Trp
Ileu Leu
His
Lys
Asp
Glu
Cys
Gly
Pro
Gln
Thr
28
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