ACE UHPLC and HPLC Columns
Amino Acids in Extracellular Matrix
(Continued) Application #AN4410
MRM extracted ion chromatograms for four amino acids each at 1 fmol on-column
Met
Tyr
Phe Thr
Reproduced with permission of SCIEX, Warrington, UK
Amino Acids Derivatised with Dabsyl Chloride
Conditions
5. L -Methionine
6. L -Isoleucine
7. L -Tryptophan
8. L -Norleucine
3
4
5
6
7
Time - Minutes
Analytes
1. L -Arginine
2. L -Threonine
3. L -Proline
4. L -Valine
1
2
9
8
Column: ACE 3 C18
Dimensions: 150 x 3.0 mm
Part Number: ACE-111-1503
Mobile Phase: A: 10 mM KH2PO4 buffer (pH 6.55)
B: MeCN/2-Propanol (70:30 v/v)
Gradient: Time (mins) %B
0.0 10
3.0 18
17.0 18
27.0 22
35.0 22
50.0 35
57.0 35
70.0 65
70.1 65
89.0 10
90.0 10
Flow Rate: 0.5 mL/min
Injection 20 μL
Temperature: 50 °C
Detection: UV, 436 nm (PDA detector)
Reproduced with permission of Department of Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
Page 2 of 2
Application #AN3420
9. L -Leucine
10. Ammonium ion
11. L -Lysine
10
11
0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0
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