Amino Acids and Biogenic Amines in Wine and Beer
Conditions
Column: ACE 5 C18-HL
Dimensions: 250 x 4.6 mm
Part Number: ACE-321-2546
Mobile Phase: A: 25 mM acetate buffer (pH 5.8)
B: MeCN/MeOH (80:20 v/v)
Gradient: Time (mins) %B
0.0 45
20.0 60
30.5 17
33.5 17
65.0 40
73.0 72
78.0 82
82.0 100
85.0 100
Flow Rate: 0.8 mL/min
Injection: 20 μL
Temperature: 16 °C
Detection: DAD, 269, 280 and 300 nm
Sample: Derivatisation with diethyl ethoxymethylmalonate
ACE UHPLC and HPLC Columns
80
Analytes
1. Aspartic acid
2. Glutamic acid
3. Asparagine
4. Serine
5. Hydroxyproline
6. Glutamine
7. Histidine
8. Glycine
9. Threonine
10. b-Alanine
11. Arginine
12. a-Alanine
Time - Minutes
Reproduced with permission of Instituto de la Vid y el Vino de Castilla-La Mancha, Spain
Application #AN2800
10
mAU
600
500
400
300
200
100
13. GABA
14. Proline
15. Histamine
16. Tyrosine
17. Ammonium ion
18. Agmatine
19. Valine
20. Methionine
21. Cysteine
22. Isoleucine
23. Tryptophan
24. Leucine
20 30 40 50 60 70
25. Phenylalanine
26. Ornithine
27. Lysine
28. Spermidine
29. Tyramine
30. Putrescine
31. Tryptamine
32. Cadaverine
33. Phenylethylamine
34. Isoamylamine
I.S. L -2-Aminoadipic acid
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specific applications or for method development support
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