Acylcarnitines by LC-MS/MS
Conditions
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL -1010-1002U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeOH
Gradient: Time (mins) %B
0.0 0.5
0.5 0.5
9.0 90.0
13.0 90.0
Flow Rate: 0.3 mL/min
Sample: Dried serum extract
Detection: Positive mode ESI
(m/z 162.16 85.25-85.35)
(m/z 204.13 85.25-85.35)
(m/z 218.13 85.25-85.35)
(m/z 232.15 85.25-85.35)
(m/z 246.20 85.25-85.35)
ACE UHPLC and HPLC Columns
(m/z 260.20 85.25-85.35)
(m/z 288.20 85.25-85.35)
(m/z 372.30 85.25-85.35)
(m/z 400.30 85.25-85.35)
Time - Minutes Time - Minutes
Application #AN1150
Analytes
1. Carnitine
2. Acetylcarnitine
3. Propionylcarnitine
4. Butyrylcarnitine & Isobutyrylcarnitine
5. Isovalerylcarnitine & 2-Methylbutyrylcarnitine
6. Hexanoylcarnitine
7. Octanoylcarnitine
8. Myristoylcarnitine
9. Palmitoylcarnitine
1
2
3
4
5
6
7
8
9
Reproduced with permission of Department of Pathology, Immunology and Laboratory Medicine, University of Florida, USA
Carnitine
Relative Abundance
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