ACE UHPLC and HPLC Columns
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Wine Acid Analysis
Application #AN1890
Conditions Analytes
1. Oxalic acid
2. Tartaric acid
3. Malic acid
4. Lactic acid
5. Ascorbic acid
6. Citric acid
Column: ACE Excel 3 C18-Amide
Dimensions: 250 x 2.1 mm
Part Number: EXL -1112-2502U
Mobile Phase: 40 mM ammonium
phosphate pH 2.5 in H2O
Flow Rate: 0.21 mL/min
Injection: 5 μL
Temperature: 25 °C
Detection: UV, 214 nm
1
2
4
5
6
1 2 3 4 5 6 7 8 9
min
mAU
20
20
17.5
17.5
15
15
12.5
12.5
10
10
7.5
5
2.5
2.5
-2.5
2 3 6 7 8 Time - Minutes
3
0
-2.5
Oxalic acid Tartaric acid Malic acid
Lactic acid Ascorbic acid Citric acid
