ACE UHPLC and HPLC Columns
2
4
5
6 1 2 3 4 5
min
1
3
175
175
150
150
125
125
100
100
75
75
50
25
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Vitamins – Water Soluble (Gradient III)
Vitamins – Water Soluble (Gradient IV)
Application #AN1880
Conditions Analytes
1. Pyridoxine
2. Pantothenic acid
3. p-Aminobenzoic acid
4. Folic acid
5. D-Biotin
6. Cyanocobalamin
7. Riboflavin
Column: ACE Ultracore 2.5
SuperPhenylHexyl
Dimensions: 50 x 2.1 mm
Part Number: CORE-25B-0502U
Mobile Phase: A: 20 mM KH2PO4, pH 2.7
B: 20 mM KH2PO4, pH 2.7 in
MeOH/H2O (50:50 v/v)
Gradient: Time (mins) %B
0.00 20
1.50 60
3.00 70
3.75 70
4.50 20
9.00 20
Flow Rate: 0.4 mL/min
Injection: 1 μL
Temperature: 40 °C
Detection: UV, 205 and 254 nm
Pyridoxine Pantothenic acid p-Aminobenzoic acid
D-Biotin
Folic acid
Riboflavin
Cyanocobalamin
1
2
6
5
4
7
Time - Minutes
Column: ACE 3 C18-AR
Dimensions: 150 x 4.6 mm
Part Number: ACE-119-1546
Mobile Phase: A: 20 mM potassium
phosphate pH 2.83 in H2O
B: 20 mM potassium
phosphate pH 2.83 in MeOH
H2O (50:50 v/v)
Gradient: Time (mins) %B
0 20
15 70
Flow Rate: 1.5 mL/min
Injection: 1 μL
Temperature: 40 °C
Detection: UV, 205 nm
Application #AN1870
Conditions Analytes
1. Pyridoxine (Vitamin B6)
2. Pantothenic acid (Vitamin B5)
3. p-Aminobenzoic acid
4. Folic acid (Vitamin B9/ Vitamin M)
5. D-Biotin (Vitamin B7/ Vitamin H)
6. Cyanocobalamin (Vitamin B12)
7. Riboflavin (Vitamin B2)
4 6 8 10 12 14 16 min
mAU
0
Pyridoxine (Vitamin B6) Pantothenic acid (Vitamin B5)
p-Aminobenzoic acid
Folic acid (Vitamin B9/ Vitamin M)
D-Biotin (Vitamin B7/ Vitamin H)
Cyanocobalamin (Vitamin B12)
Riboflavin (Vitamin B2)
0 2 6 10 12 14 16
Time - Minutes
0
3
1
2
4
5
6 7
R=CN
R=CN
mAU