ACE UHPLC and HPLC Columns
Vitamins – Fat Soluble
1200
1000
800
600
400
200
180
2
1
11
2
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Application #AN2420
Conditions Analytes
1. Menadione (Vitamin K3)
2. Retinol (Vitamin A)
3. Vitamin A acetate
4. Ergocalciferol (Vitamin D2)
5. Cholecalciferol (Vitamin D3)
6. Vitamin E acetate
7. α-Tocopherol (Vitamin E)
8. Vitamin K1
Column: ACE Excel 3 C18-Amide
Dimensions: 150 x 4.6 mm
Part Number: EXL -1112-1546U
Mobile Phase: MeOH/MeCN (90:10 v/v)
Flow Rate: 1 mL/min
Temperature: 20 °C
Detection: UV, 280 nm Menadione (Vitamin K3) Retinol (Vitamin A)
Vitamin A acetate
Ergocalciferol (Vitamin D2) Cholecalciferol (Vitamin D3)
Vitamin E acetate
α-Tocopherol (Vitamin E)
6
4 5
7
3
2 4 6 8
8
Vitamin K1 Time - Minutes
Vitamins – Water Soluble (Gradient I)
Application #AN2940
Conditions Analytes
1. Pyridoxamine
2. Thiamine (Vitamin B1)
3. L -Ascorbic acid (Vitamin C)
4. Niacinamide (Vitamin B3)
5. Nicotinic acid
6. Pyridoxal
7. Pyridoxine
8. p-Aminobenzoic acid
9. Pantothenic acid (Vitamin B5)
10. Folic acid (Vitamin B9)
11. Cyanocobalamin (Vitamin B12)
12. Riboflavin (Vitamin B2)
13. d-Biotin (Vitamin B7)
14. Thioctic acid
Column: ACE 5 C8
Dimensions: 250 x 4.6 mm
Part Number: ACE-122-2546
Mobile Phase: A: 50 mM KH2PO4 pH
2.5 in H2O
B: MeOH
Gradient: Time (mins) %B
0.0 0
3.0 0
16.5 45
19.5 80
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 205 nm
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
Time - Minutes
0
0
6 7
8
9 10
12
13
14
4
5
3
2
1
Vitamins – Water Soluble (Gradient II)
Application #AN2930
Conditions Analytes
1. Pyridoxamine
2. Thiamine (Vitamin B1)
3. L -Ascorbic acid (Vitamin C)
4. Nicotinic acid
5. Pyridoxal
6. Impurity
7. Pyridoxine
8. p-Aminobenzoic acid
9. Pantothenic acid (Vitamin B5)
10. Folic acid (Vitamin B9)
11. Cyanocobalamin (Vitamin B12)
12. d-Biotin (Vitamin B7)
13. Riboflavin (Vitamin B2)
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: A: 50 mM KH2PO4
pH 3.0 in H2O
B: MeOH
Gradient: Time (mins) %B
0 3
5 3
45 45
50 80
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 205 nm
1200
200
0
0
5 10 15 20 25 30 35 40 45
Time - Minutes
1000
800
600
400
3
1
4
5 6
7
8
9
10,11
12
13
Response - MilliVolts
Response - MilliVolts