ACE UHPLC and HPLC Columns
10
8
4
2
0
80
80
60
60
40
40
20
0
174
2
1
Hydrocortisone
Propylparaben
0 0.5 1 1.5 2
2.5 3 3.5 4 4.5 min
mAU
120
100
80
80
60
60
40
40
20
20
0
Guaifenesin
Benzoic acid
2
0.25 0.5 0.75 1 1.25 1.5 1.75 min
0.25 0.5 0.75 1 1.25 1.5 1.75
0
200
200
150
150
100
100
50
50
0
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1
2
2.5 3 4 4.5
Time - Minutes Time - Minutes
mAU
6
1
2
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 4.6 mm
Part Number: CORE-25A-1046U
Mobile Phase: H2O/MeCN/MeOH
(50:25:25 v/v/v)
Flow Rate: 1 mL/min
Injection: 5.8 μL
Temperature: Ambient (22 °C)
Detection: UV, 254 nm
USP Monograph – Guaifenesin
Application #AN1750
Conditions Analytes
1. Guaifenesin
2. Benzoic acid
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 50 x 3.0 mm
Part Number: CORE-25A-0503U
Mobile Phase: H2O/MeOH/Glacial acetic acid
(60:40:1.5 v/v/v)
Flow Rate: 0.85 mL/min
Injection: 1.5 μL
Temperature: Ambient (22 °C)
Detection: UV, 276 nm
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
USP Monograph – Hydrocortisone
Application #AN1770
Conditions Analytes
1. Hydrocortisone
2. Propylparaben
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
USP Monograph – Hydroquinone
Application #AN1780
Conditions Analyte
Column: ACE Excel 2 C18 1. Hydroquinone
Dimensions: 50 x 3.0 mm
Part Number: EXL -101-0503U
Mobile Phase: H2O/MeOH (45:55 v/v)
Flow Rate: 0.45 mL/min
Injection: 0.9 μL
Temperature: Ambient (22 °C)
Detection: UV, 280 nm
USP Monograph – Indomethacin
Application #AN1790
Conditions Analyte
Column: ACE 5 C18 1. Indomethacin
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 0.01 M monobasic sodium
phosphate and 0.01 M dibasic
sodium phosphate in
MeCN/H2O (1:1 v/v)
Flow Rate: 1.32 mL/min
Injection: 13 μL
Temperature: Ambient (22 °C)
Detection: UV, 254 nm
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
1
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
0.25 0.75 1 1.5 1.75 min
mAU
0
1
0.25 0.5 0.75 1 1.25 1.5 1.75
20
0 1 3 4 min
mAU
0
0 1 2 3 4
Time - Minutes Time - Minutes