ACE UHPLC and HPLC Columns
2
5 10 15 20 25 min
mAU
17.5
17.5
15
15
12.5
12.5
10
10
7.5
7.5
2.5
2.5
0
Time - Minutes Time - Minutes
Application #AN1740
1. Ethylparaben
2. Estradiol
3. Estrone
USP Monograph – Glimepiride
Ethylparaben
Estradiol
Estrone
1
14
Glimepiride
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USP Monograph – Budesonide
Application #AN1720
Conditions Analytes
1. Budesonide B
2. Budesonide A
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 18 mM monobasic sodium
phosphate pH 3.2 in
H2O/MeCN (68:32 v/v)
Flow Rate: 1.5 mL/min
Injection: 20 μL
Temperature: Ambient (22 °C)
Detection: UV, 254 nm
mAU
160
160
140
140
120
120
100
100
80
80
60
60
40
20
0
0
1
2
40
20
5 10 15 20
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
USP Monograph – Doxepin
Application #AN1730
Conditions Analytes
1. Doxepin isomer
2. Doxepin isomer
Column: ACE 3 C8
Dimensions: 50 x 4.6 mm
Part Number: ACE-112-0546
Mobile Phase: 0.2 M monobasic NaH2PO4
pH 2.5 in H2O/MeOH (30:70 v/v)
Flow Rate: 0.56 mL/min
Injection: 4.5 μL
Temperature: 50 °C
Detection: UV, 254 nm
1
2
10 15 20 25
5
0
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
USP Monograph – Estradiol
Conditions Analytes
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 4.6 mm
Part Number: CORE-25A-1046U
Mobile Phase: H2O/MeCN (45:55 v/v)
Flow Rate: 1.39 mL/min
Injection: 10.1 μL
Temperature: Ambient (22 °C)
Detection: UV, 205 nm
Time - Minutes Time - Minutes
Application #AN1760
Conditions Analyte
Column: ACE 5 C18 1. Glimepiride
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 8 mM monobasic sodium
phosphate pH 2.4/MeCN (1:1 v/v)
Flow Rate: 1.32 mL/min
Injection: 16 μL
Temperature: Ambient (22 °C)
Detection: UV, 228 nm
0 0.5 1 1.5 2 2.5 3
3.5 4
4.5 min
mAU
mAU
200
200
150
150
100
100
50
0
1 2
3
0
1 2
3
0.5 1.5 2.5 3.5 4.5
50
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
2 4 6 8 10 12 14 min
Translated method according to USP <621> guidance. Always check the
latest and official method information from the relevant pharmacopoeia prior
to analysis.
mAU
mAU