ACE UHPLC and HPLC Columns
Tocopherols
600
500
400
300
200
100
Tricyclic Antidepressants
4
Conditions Analytes
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: MeOH/25 mM KH2PO4 pH 6.0
in H2O (80:20 v/v)
Flow Rate: 1 mL/min
Temperature: 22 °C
Detection: UV, 215 nm
1
2
3
4 5
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
168
Application #AN3920
1. Norephedrine
2. Nortriptyline
3. Toluene
4. Imipramine
5. Amitriptyline
Time - Minutes
Toxins from Amanita Phalloides Mushrooms
by LC-HRMS Application #AN4060
Conditions
Column: ACE 3 AQ
Dimensions: 150 x 3.0 mm
Part Number: ACE-116-1503
Mobile Phase: A: 0.1% formic acid
in H2O
B: 0.1% formic acid
in MeOH
Gradient: Time (mins) %B
0 15
17 100
22 100
22 15
30 15
Flow Rate: 0.4 mL/min
Injection: 10 μL
Temperature: 50 °C
Detection: Thermo Exactive MS
ESI in positive ion mode
Amanita Phalloides
Peak Analyte R1 R2 R3 R4 R5 Exact Mass
1 a - Amanitin OH OH NH2 OH OH 918.35417
2 b - Amanitin OH OH OH OH OH 919.338182
3 g - Amanitin H OH NH2 OH OH 902.359252
4 Amaninamide OH OH NH2 H OH 902.359252
5 Amanin OH OH OH H OH 903.343267
6 e - Amanitin H OH OH OH OH 903.343267
7 Amanullin H H NH2 OH OH 886.364337
8 Proamanullin H H NH2 OH H 870.369423
9 Amanullinic Acid H H OH OH OH 887.348353
10 Bacitracin (IS) - - - - - 1421.7489
100 1
50
0
100
50
0
100
50
0
100
50
0
100
50
0
100
50
0
100
50
0
100
50
0
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Analyte
1. Amatoxins
7
9
10
4
5
6
6
8
3
3
2
2
4
5
2 4 6 8 10 12 14
Reproduced with permission of Fera Science Ltd, York, UK
8
9
10
7
Time - Minutes
Application #AN2790
Conditions
Column: ACE 5 SIL
Dimensions: 250 x 4.6 mm
Part Number: ACE-127-2546
Mobile Phase: Hexane/IPA (98:2 v/v)
Flow Rate: 1 mL/min
Injection: 1 μL
Temperature: Ambient
Detection: UV-Vis, 450 nm
1. γ-Tocopherol
2. α-Tocopherol
3. β-Tocopherol
4. β-Tocopherol
5. δ-Tocopherol
1
2
3
5
0 1 2 3 4 5 6 7 8 9 10
0
Analytes
Time - Minutes
Response - MilliVolts