ACE UHPLC and HPLC Columns
240
220
200
180
160
140
120
100
80
60
40
20
Response - MilliVolts
166
100
80
80
60
60
40
40
20
20
1 2
Time - Minutes Time - Minutes
Tetracyclines
Demeclocycline Chlortetracycline Doxycycline
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2
100
0
100
100
80
80
60
60
40
40
20
20
0
0
0 2 4 6 8 0 2 4 6 8
mins
Terfenadine and Fexofenadine in Rat Plasma
Application #AN3290
Conditions Analytes
1. Fexofenadine
(m/z 502.3 466.3)
2. Terfenadine
(m/z 472.3 436.3)
Column: ACE 5 AQ
Dimensions: 50 x 3.0 mm
Part Number: ACE-126-0503
Mobile Phase: A: 0.1% formic acid in H2O
B: MeOH
Gradient: Time (mins) %B
0.0 10
1.5 90
2.0 90
3.0 10
Flow Rate: 1 mL/min
Injection: 10 μL
Temperature: Ambient
Detection: TurboIonSpray MS/MS
Positive ion mode
0
0
2 4 6 8 0
2 4 6 8
Reproduced with permission of Aptuit, Edinburgh, UK
Fexofenadine Terfenadine
Testosterone
Application #AN3300
Conditions Analyte
Column: ACE 5 C18 1. Testosterone
Dimensions: 150 x 0.075 mm
Part Number: ACE-121-1500075
Mobile Phase: A: 0.1% formic acid in
MeCN/0.1% formic acid in H2O (10:90 v/v)
B: 0.1% formic acid in MeCN/0.1%
formic acid in H2O (90:10 v/v)
Gradient: Time (mins) %B
0 40
5 40
30 95
Flow Rate: 1 μL/min
Temperature: Ambient
Detection: ESI MS/MS
Positive ion mode
Testosterone
0 2 4 6 8 10 12 14 16 18
0
Oxytetracycline Tetracycline
Time - Minutes Time - Minutes
Application #AN3680
Conditions Analytes
1. Oxytetracycline
2. Tetracycline
3. Demeclocycline
4. Chlortetracycline
5. Doxycycline
Column: ACE 5 C18
Dimensions: 150 x 3.0 mm
Part Number: ACE-121-1503
Mobile Phase: 10 mM oxalic acid pH 2.9/MeCN
(80:20 v/v)
Flow Rate: 0.5 mL/min
Injection: 20 μL
Temperature: Ambient
Detection: UV-Vis, 350 nm
2
1
1
3
4
5
0 5 10 15
Reproduced with permission of University of London, UK Reproduced with permission of Fera Science Ltd, York, UK