ACE UHPLC and HPLC Columns
Synthetic Cannabinoids (SPICE) from Oral Fluid
Column: ACE Excel 2 C18-AR
Dimensions: 100 x 2.1 mm
Part Number: EXL -109-1002U
Mobile Phase: 0.1% formic acid in
MeOH/H2O (85:15 v/v)
Flow Rate: 0.3 mL/min
Temperature: Ambient
Detection: Applied Biosystems/MDS
Sciex 4000 Q-Trap
Positive mode Turbo Ionspray
1.7e5
1.6e5
1.5e5
1.4e5
1.3e5
1.2e5
1.1e5
1.0e5
9.0e4
8.0e4
7.0e4
6.0e4
5.0e4
4.0e4
3.0e4
2.0e4
164
UR - 144 JWH - 200
Extracted ion chromatogram for SPICE analytes
fortified in neat oral fluid at 20 ng/mL
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Application #AN1650
Conditions
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5
1.0e4
0.0
Time - Minutes
Retention
Time (minutes)
Analyte MRM
Transition
Declustering
Potential (DP)
Collision
Energy (CE)
Cell Exit
Potential (CXP)
2.55 JWH-250 N-(5-hydroxypentyl) 352 120.9 40 30 16
2.99 JWH-073 N-(3-hydroxybutyl) 344 155 40 30 16
3.00 UR-144 5-Hydroxy-pentyl 328.5 125 30 35 16
3.03 UR-144 Pentanoic Acid 342.5 125 30 35 16
3.14 d5-JWH-018 N- (4-hydroxypentyl) 363.5 155 40 35 16
3.14 JWH-018 N- (4-hydroxypentyl) 358 155 40 30 16
3.34 JWH-018 5-pentanoic acid 372 155 40 30 16
3.98 JWH-200 385 155 40 30 16
4.69 XLR-11 330 125 30 35 16
5.32 JWH-250 336 121 40 30 16
6.36 JWH-073 328 155 40 30 16
6.37 UR-144 5-Chloro-pentyl 346.9 125 30 35 16
6.55 UR-144 312.5 125 30 35 16
8.14 JWH-018 342 155 40 30 16
Reproduced with permission of Biotage®, Charlotte, NC, USA. See Biotage Application note AN791 for further details of extraction & analysis.
Intensity (cps)