ACE UHPLC and HPLC Columns
Sulfurous Analytes Separation Comparison
Column: ACE 3 C18-AR, ACE 3 C18
Dimensions: 50 x 2.1 mm
Part Number: ACE-119-0502, ACE-111-0502
Mobile Phase: A: H2O
B: MeOH
Gradient: Time (mins) %B
0.0 30
5.0 30
9.0 95
9.5 30
13.5 30
Flow Rate: 0.5 mL/min
Injection: 1 μL
Temperature: 22 °C
Detection: UV, 254 nm
162
1
2
1,2
Methyl phenyl sulfoxide Methyl phenyl sulfone
1
1.5e6
1.4e6
1.3e6
1.2e6
1.1e6
1.0e6
9.0e5
8.0e5
7.0e5
6.0e5
5.0e5
4.0e5
3.0e5
2.0e5
Time - Minutes
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com
Application #AN1320
Conditions Analytes
1. Methyl phenyl sulfoxide
2. Methyl phenyl sulfone
3. Toluene
4. Thioanisole
4
4
3
3
ACE C18-AR
ACE C18
0 1 2 3 4 5 6 7 8
Toluene Thioanisole
Sumatriptan and Promethazine by LC-MS/MS
Application #AN2530
Conditions Analytes
1. Sumatriptan
(m/z 296 58)
2. Promethazine
(m/z 286 86)
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL -1010-1002U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeOH
Gradient: Time (mins) %B
0.0 15
0.5 15
2.5 90
3.5 90
5.5 15
Flow Rate: 0.7 mL/min
Temperature: 50 °C
Detection: AB Sciex QTRAP 6500
DuoSpray Ion source (ESI/APCI)
Positive ion MRM mode
Sumatriptan Promethazine
0.0
2
1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6
1.0e5
Reproduced with permission of Charles River Montreal, Canada
Time - Minutes