ACE UHPLC and HPLC Columns
Sugars Separation
Column: ACE Excel 5 NH2
Dimensions: 150 x 4.6 mm
Part Number: EXL - 1214-1546U
Mobile Phase: MeCN/H2O (70:30 v/v)
Flow Rate: 1 mL/min
Injection: 10 μL
Temperature: 35 °C
Detection: RI, 35 °C
uRIU
70
60
50
40
30
20
10
1
4
3
70
60
50
40
30
20
10
2
5
6
7
8
9
Application #AN4160
uRIU
uRIU
50
50
40
40
30
30
20
20
10
10
2
1
3
0
2 4 6 8 10 12 14 min
2 4 6 8 10 12 14 16
4 6 8 10 12 14
mAU
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40
40
35
30
30
25
20
15
15
10
5
mins
35
Sugars – Orange Juice
Conditions Analytes
Column: ACE Excel 3 NH2
Dimensions: 150 x 4.6 mm
Part Number: EXL -1114-1546U
Mobile Phase: MeCN/H2O (75:25 v/v)
Flow Rate: 1 mL/min
Injection: 10 μL
Temperature: 35 °C
Detection: RI, 35 °C
0
0
Sulfonamides
1. Fructose
2. Glucose
3. Sucrose
Time - Minutes
Column: ACE Excel 3 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: EXL -1110-1546U
Mobile Phase: A: H2O
B: MeCN
C: 10% formic acid
Gradient: Time (mins) %A %B %C
0 84 15 1
12 74 25 1
14 59 40 1
16 84 15 1
18 84 15 1
Flow Rate: 1 mL/min
Detection: UV, 268 nm
Application #AN1610
Conditions Analytes
1. Sulfadiazine
2. Sulfapyridine
3. Sulfamerazine
4. Sulfamoxole
5. Sulfamethazine
6. Sulfamonomethoxine
7. Sulfachloropyridazine
8. Sulfamethoxazole
9. Sulfadimethoxine
2 4 6 8 10 12 14 16
10
8
6
1
3
5
7
Time - Minutes
Application #AN4030
Conditions Analytes
1. Fructose
2. Sucrose
3. Lactose
2 4 12 14 min
uRIU
0
0 2 4 6 8 10 12 14
0
2
3
1
Time - Minutes
Reproduced with permission of National Research Institute of Agriculture and Food Technology INIA, Spain