ACE UHPLC and HPLC Columns
Application #AN2430
1. 1,2,3-Trimethoxybenzene
2. 1,2-Dimethoxybenzene
3. 1,2,4-Trimethoxybenzene
4. 1,4-Dimethoxybenzene
5. Methoxybenzene
6. 1,3-Dimethoxybenzene
7. 1,3,5-Trimethoxybenzene
8. Toluene
1,2,3-Trimethoxybenzene 1,2-Dimethoxybenzene 1,2,4-Trimethoxybenzene
1,4-Dimethoxybenzene Methoxybenzene 1,3-Dimethoxybenzene
1,3,5-Trimethoxybenzene Toluene
Sugars – Cola vs Diet Cola
uRIU
70
70
60
50
50
40
40
30
30
20
20
10
10
0
0
-10
70
70
60
60
50
50
40
40
30
30
20
20
10
0
0
Time - Minutes Time - Minutes
Sugars – Lactulose
uRIU
35
35
30
30
25
25
20
20
15
15
10
10
5
5
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 159
Substituted Methoxybenzene Isomers
Conditions Analytes
Column: ACE 3 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: ACE-1110-1546
Mobile Phase: H2O/MeOH (50:50 v/v)
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 40 °C
Detection: UV, 214 nm
1
2
3
4 5
6
8
4 6
7
5 10 15 20 min
5 10 15 20
Column: ACE Excel 3 NH2
Dimensions: 150 x 4.6 mm
Part Number: EXL -1114-1546U
Mobile Phase: MeCN/H2O (75:25 v/v)
Flow Rate: 1 mL/min
Injection: 10 μL
Temperature: 35 °C
Detection: RI, 35 °C
Application #AN4150
Conditions Analytes
1. Fructose
2. Glucose
3. Sucrose
0 2 4 6 8 10 12 14 min
uRIU
-10
0 2 4 6 8 10 12 14 min
uRIU
-10
Diet Cola
0 2 4 6 8 10
8
12 14
0 2 4 6 10 12 14
-10
1 2
3
Cola
Diet Cola
Column: ACE Excel 5 NH2
Dimensions: 150 x 4.6 mm
Part Number: EXL -1214-1546U
Mobile Phase: MeCN/H2O (70:30 v/v)
Flow Rate: 1 mL/min
Injection: 10 μL
Temperature: 35 °C
Detection: RI, 35 °C
Application #AN4020
Conditions Analytes
1. Galactose
2. Lactulose
3. Lactose
1
2
0 2.5 5 7.5 10 12.5 15 17.5 min
uRIU
0
3
2.5 5 7.5 10 12.5 15 17.5
0
Time - Minutes
uRIU
Sugars – Disaccharides
Conditions
uRIU
20
15
10
5
0
-10
Analytes
1 . Sucrose
2. Maltose
3. Lactose
0 5 10 15 20 25 min
-5
Time - Minutes
Application #AN4320
Column: ACE Excel 3 NH2
Dimensions: 150 x 4.6 mm
Part Number: EXL -1114-1546U
Mobile Phase: MeCN/H2O (80:20 v/v)
Flow Rate: 1 mL/min
Injection: 20 μL
Temperature: 35 °C
Detection: RI, 35 °C
Sample: 2 mg/mL each disaccharide in MeCN/H2O (80:20 v/v)
System: Chromaster 600
3
1
2