ACE UHPLC and HPLC Columns
St John’s Wort
Conditions
mAU
40
30
20
10
0
mAU
40
30
20
10
0
mAU
40
30
20
10
158
RS = 2.66
Time - Minutes
RS = 1.53
RS = 2.10
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Application #AN4300
Column: ACE Excel 1.7 SuperC18
Dimensions: 100 x 3.0 mm; 2 x 100 x 3.0 mm (coupled); 3 x 100 x 3.0 mm (coupled)
Part Number: EXL -1711-1003U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins)
100 x 3.0 mm 200 x 3.0 mm 300 x 3.0 mm %B
- 0.00 0.00 5
0.00 0.35 0.71 5
15.00 30.35 45 .71 30
17 .00 34.35 51 .71 100
19.00 38.35 57 .71 100
20.00 39.35 58.71 5
27 .00 53.35 79.71 5
Flow Rate: 0.8 mL/min
Injection: 2 μL (100 x 3.0 mm); 4 μL (200 x 3.0 mm); 6 μL (300 x 3.0 mm)
Temperature: 80 °C
Detection: UV, 280 nm
Sample: Tablet ground to fine powder and extracted with MeCN/H2O (1:1 v/v) with
ultrasonication. Supernatant diluted with H2O and filtered using Whatman
Mini-Uniprep syringeless filter
System: Chromaster Ultra Rs
100 x 3.0 mm
200 x 3.0 mm
300 x 3.0 mm
Hypericum perforatum
0
0
10 20 30 40 50