ACE UHPLC and HPLC Columns
45
40
35
30
25
20
15
10
5
Statins – Pravastatin
65
60
55
50
45
40
35
30
25
20
15
10
5
Steroid Hormones (Endogenous) by LC-MS/MS
Application #AN2640
Conditions Analytes
1. Aldosterone
(m/z 361.4 315.4)
2. 21-Deoxycortisol
(m/z 347.4 311.4)
3. 11-Deoxycortisol
(m/z 347.4 97.0)
1
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
2
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
3
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
4
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
5
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
6
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
7
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
8
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
9
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Column: ACE Excel 2 C18
Dimensions: 50 x 2.1 mm
Part Number: EXL -101-0502U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeOH
Gradient: Time (mins) %B
0.0 50
0.8 50
4.2 81
4.3 100
5.3 100
5.8 50
6.8 50
Flow Rate: 0.4 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: MS/MS
ESI in positive ion mode
Reproduced with permission of Biotage GB Ltd, UK
Application #AN3330
Conditions Analyte
Column: ACE 5 C18 1. Pravastatin
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 10 mM ammonium formate
pH 3.0/MeCN (68:32 v/v)
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: Ambient
Detection: UV, 254 nm
Pravastatin
0 1 2 3 4 5 6 7 8 9 10
Time - Minutes
0
1
Statins – Simvastatin
Application #AN3340
Conditions Analyte
Column: ACE 5 C18 1. Simvastatin
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 10 mM ammonium formate
pH 3.0/MeCN (25:75 v/v)
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: Ambient
Detection: UV, 254 nm
Simvastatin
0 1 2 3 4 5 6 7 8 9 10
Time - Minutes
0
1
4. Androstenedione
(m/z 287.3 97.0)
5. Testosterone
(m/z 289.4 97.0)
6. DHEA
(m/z 289.4 253.2)
7. 17α-Hydroxyprogesterone
(m/z 331.4 97.0)
8. Progesterone
(m/z 315.4 97.0)
9. Androsterone
(m/z 291.3 255.4)
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
100
%
0
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
Time - Minutes
Response - MilliVolts
Response - MilliVolts