ACE UHPLC and HPLC Columns
160
140
120
100
80
60
40
20
154
350
300
250
200
150
100
50
ACE
HPLC and UHPLC Columns
Independent Column Comparisons
ACE UltraCore SuperC18 Provides Exceptional Efficiency
- Reproduced with kind permission of The Open University, UK.
■ Leading column brands from major manufacturers investigated
■ Comparison of column efficiency for pyridine – a basic molecule
Peak Efficiency Comparison Application # 1802
ACE UltraCore 2.5 SuperC18
Phenomenex Kinetex 1.3 C18
Phenomenex Kinetex 1.7 C18
Waters Cortecs 1.6 C18
Phenomenex Kinetex 2.6 C18
Thermo Accucore 2.6 C18
Pressure Efficiency
117 bar
450 bar
235 bar
285 bar
157 bar
117 bar
49,100pl/m
44,400pl/m
35,600pl/m
35,200pl/m
34,800pl/m
19,200pl/m
0 20,000 40,000 60,000 80,000
N0.5 (pyridine) (plates/m)
Higher Efficiency
Lower Efficiency
“Independent Testing Shows ACE Solid-Core Columns
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com
Statins – Atorvastatin
Application #AN3310
Conditions Analyte
Column: ACE 5 C18 1. Atorvastatin
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 10 mM ammonium formate
pH 3.0/MeCN (47:53 v/v)
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: Ambient
Detection: UV, 254 nm
Atorvastatin
0 1 2 3 4 5 6 7 8 9 10
Time - Minutes
0
1
Statins – Fluvastatin
Application #AN3320
Conditions Analyte
Column: ACE 5 C18 1. Fluvastatin
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 10 mM ammonium formate
pH 3.0/MeCN (47:53 v/v)
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: Ambient
Detection: UV, 254 nm
Fluvastatin
0 1 2 3 4 5 6 7 8 9 10
Time - Minutes
0
1
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UHPLC and HPLC Columns
Independent Testing Comparison #3: Solid-Core Particles
Column Dimensions: 50 x 2.1mm Sample: 1) uracil 2) pyridine 3) phenol
Mobile Phase: 30:70 (v/v) MeOH/10mM NH4OAc in H2O (pH 5.8) Flow Rate: 0.20ml/min Temperature: 22°C Wavelength: 254nm
Comparative data may not be representative of all applications.
■ Improved efficiency and peak shape has a direct effect upon signal response
Reduced
Tailing -
Increased
Increased Peak Height
Tailing -
Reduced
Peak Height
Increased
Response
Improve your MS signal
response by selecting
high efficiency, low
tailing ACE UHPLC and
HPLC Columns
• UHPLC and HPLC columns
• Porous and solid-core particles
• Asymmetry and efficiency comparisons
• Independently tested for impartial results
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Response - MilliVolts
Response - MilliVolts