ACE UHPLC and HPLC Columns
1000 Sennoside B Sennoside A
Sennoside B
1,000
800
800
600
600
400
400
200
200
0
0
-200
-200
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0
2.25
2.25
2.00
2.00
1.75
1.75
1.50
1.50
1.25
1.25
1.00
1.00
0.75
0.75
0.50
0.50
0.25
0.25
0.00
-0.25
mAU(x100)
AD1:214nm
3
Crotamine is a 42 residue
long cationic polypeptide
containing 11 basic
residues (9 lysines, 2
arginines) and 6 cysteines
0.0 2.5 5.0 7.5 10.0 min
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 151
-400
mAU
min
Absorbance mAU
Retention Time min
2
1
Sennoside B
Sennoside A
Sample
Standard
Sennosides in Traditional Chinese Medicine
Application #AN1390
Conditions Analytes
Sennoside A
Sennoside B
Column: ACE 3 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: ACE-1110-1546
Mobile Phase: A: 0.75% acetic acid in H2O
B: MeCN/MeOH (90:10 v/v)
Gradient: Time (mins) %B
0 9
23 28
40 28
Flow Rate: 0.6 mL/min
Temperature: 35 °C
Detection: UV, 271 nm
Sample: Herbal tea bag containing
Folium Sennae, Peppermint,
Folium Mori, Folium Nelumbinis,
Glycyrrhiza Uralensis and
Lalang Grass Rhizome
Sennoside A
Time - Minutes
Sample
-400
Reproduced with permission of Vercotech Inc., Taiwan
Snake Venom from Crotalus Durissus Terrificus
Application #AN4190
Conditions Analytes
1. Crotamine
2. Crotapotin
3. Phospholipase A2 (PLA2)
Column: ACE 3 C8-300
Dimensions: 50 x 4.6 mm
Part Number: ACE-212-0546
Mobile Phase: A: 0.1% TFA in H2O
B: 0.1% TFA in MeCN/H2O (90:10 v/v)
Gradient: Time (mins) %B
0 20
2 20
27 70
Flow Rate: 1.2 mL/min
Injection: 20 μL
Detection: UV, 214 nm
Sample: Lyophilised crude venom powder was solubilised (1 mg/mL)
in 0.1% TFA. Resulting solutions were centrifuged
and the supernatant used for analyses.
Time - Minutes
0
-0.25
5.0 7.5
2
2.5 10.0
5
1
0.00
Sotalol
Application #AN3700
Conditions Analyte
Column: ACE 5 CN 1. Sotalol
Dimensions: 150 x 4.6 mm
Part Number: ACE-124-1546
Mobile Phase: 20 mM ammonium formate
pH 3.0/MeOH (85:15 v/v)
Flow Rate: 1 mL/min
Injection: 20μL (0.2mg/mL solution)
Temperature: Ambient
Detection: UV, 254 nm
Sotalol
1
Time - Minutes
0.05
0.045
0.04
0.035
0.03
0.025
0.02
0.015
0.01
0
0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
0.005
Reproduced with permission of School of Pharmacy, University of
Sunderland, UK
Reproduced with permission of Center for the Study of Venoms and
Venomous Animals, (CEVAP), UNESP, Sao Paulo State University, Brazil