ACE UHPLC and HPLC Columns
Ranitidine hydrochloride
Sharma N, Rao S, Kumar N, Reddy P, Reddy A (2011) A Validated Stability-Indicating Liquid-Chromatographic Method for Ranitidine Hydrochloride in Liquid Oral
Dosage Form. Sci Pharm. 79, 309. doi:10.3797/scipharm.1101-06
Recombinant hGMCSF Purified from Escherichia Coli
350000
300000
250000
200000
150000
100000
50000
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 149
1
2
3
6
7
8
Ranitidine Hydrochloride and Related Impurities
Application #AN3450
Conditions Analytes
1. Impurity F
2. Impurity E
3. Impurity D
4. Impurity A
5. Impurity C
6. Impurity G
7. Ranitidine
8. Impurity B
5
7
5
4
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00
0.040
0.030
0.020
0.010
0.000
Time - Minutes
Time - Minutes
Column: ACE 3 C18
Dimensions: 100 x 4.6 mm
Part Number: ACE-111-1046
Mobile Phase: A: 0.05 M KH2PO4 pH 6.5 in H2O/MeCN (98:2 v/v)
B: H2O/MeCN (5:95 v/v)
Gradient: Time (mins) %B
0 0
10 5
25 15
35 20
40 55
55 0
Flow Rate: 1 mL/min
Injection: 40 μL
Temperature: 40 °C
Detection: UV, 230 nm
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: A: 0.1% TFA in H2O/MeCN (90:10 v/v)
B: 0.1% TFA in H2O/MeCN (10:90 v/v)
Gradient: Time (mins) %B
0 10
20 65
23 100
Flow Rate: 1 mL/min
Temperature: 30 °C
Detection: UV, 215 nm
hGMCSF = human Granulocyte Macrophage Colony
Stimulating Factor, a 127 amino acid residue cytokine
with a molecular weight of 14,477 Da
Application #AN3840
Conditions
10.0 15.0 20.0 25.0 30.0 35.0
0
Das KMP, Banerjee S, Shekhar N, Damodaran K, Nair R, Somani S, Raiker VP, Jain S, Padmanabhan S. Cloning, Soluble Expression and Purification of High Yield
Recombinant hGMCSF in Escherichia coli. Int. J. Mol. Sci. 2011, 12, 2064-2076; doi:10.3390/ijms12032064