ACE UHPLC and HPLC Columns
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Prostaglandins using LC-MS/MS
Application #AN3260
Conditions Analytes
Column: ACE 3 C18
Dimensions: 50 x 2.1 mm
Part Number: ACE-111-0502
Mobile Phase: A: H2O
B: MeOH
C: MeCN
Gradient: Time (mins) %A %B %C
0 70.0 20.0 10.0
9 10.0 60.0 30.0
10 0.1 66.6 33.3
Flow Rate: 0.2 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: ESI (-) MS/MS
MRM m/z 353.3 193
1. 8-iso-Prostaglandin F2α
2. Prostaglandin F2α
8-iso-Prostaglandin F2α Prostaglandin F2α
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2
0 1 2 3 4 5 6 7 8 9 10 11 12
0
Reproduced with permission of Department of Internal Medicine, Fukuoka University, Japan
Time - Minutes
Protein Test Mix
Application #AN3730
Conditions Analytes
1. Ribonuclease A (MW ~14 kDa)
2. Cytochrome C (MW ~12 kDa)
3. Holo-transferrin (MW ~77 kDa)
4. Apomyoglobin (MW ~17 kDa)
Column: ACE 5 C18-300
Dimensions: 250 x 4.6 mm
Part Number: ACE-221-2546
Mobile Phase: A: 0.1% TFA in H2O
B: 0.1% TFA in MeCN
Gradient: Time (mins) %B
0 5
30 70
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 280 nm
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Time - Minutes
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0
Proton Pump Inhibitors (PPIs)
Application #AN3710
Conditions Analytes
1. Omeprazole
2. Lansoprazole
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 10 mM ammonium formate
pH 3.0/MeCN (65:35 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
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2
0 1 2 3 4 5 6 7 8 9 10
Time - Minutes
0
Omeprazole
Lansoprazole
Response - MilliVolts
Response - MilliVolts
Response - MilliVolts