ACE UHPLC and HPLC Columns
Porphyrins in Oral Bacteria by LC-MS/MS
Column: ACE 3 C18-PFP
Dimensions: 75 x 2.1 mm
Part Number: ACE-1110-7502
Mobile Phase: A: 0.1% formic acid in H2O/MeCN (95:5 v/v)
B: 0.1% formic acid in H2O/MeCN (5:95 v/v)
Gradient: Time (mins) %B
0.0 30
10.0 50
10.2 100
35.0 100
Flow Rate: 0.1 mL/min
Injection: 5 μL
Temperature: 25 °C
Detection: SCIEX API 2000 triple quad MS
TurbolonSpray interface
Positive ESI
Ion source temperature: 200 °C
Capillary voltage: 5 kV
2
1
4
5 6
7
8
3
100%
50%
Reproduced with permission of Department of Environmental Science and Analytical Chemistry, Stockholm University, Sweden
Pravastatin and Isomers by LC-MS/MS
Column: ACE 3 C18
Dimensions: 50 x 3.0 mm
Part Number: ACE-111-0503
Mobile Phase: MeCN/MeOH/THF/H2O/Acetic Acid
(15:20:5:60:0.1 v/v/v/v/v)
Flow Rate: 0.6 mL/min
Injection: 2 μL
Temperature: Ambient
Detection: API 3000 triple quad MS
TurbolonSpray – negative mode
Extracted ion chromatogram
of MRM m/z 423.3 321.1
142
1. 6-epi-Pravastatin
(MW 424)
2. Pravastatin
(MW 424)
3. 3a-iso-Pravastatin
(MW 424)
6-epi-Pravastatin
Pravastatin
3a-iso-Pravastatin
8.5e4
8.0e4
7.5e4
7.0e4
6.5e4
6.0e4
5.5e4
5.0e4
4.5e4
4.0e4
3.5e4
3.0e4
2.5e4
2.0e4
1.5e4
1.0e4
5000.0
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Application #AN1350
Conditions Analytes
Reproduced with permission of Biotrial Bioanalytical Services, Laval, QC, Canada
2
3
1
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8
0.0
Time - Minutes
1 2
3
4
5 6
7
8
2 4 6 8 10 12 14 16 18 20 22 24 26 28
0%
Time - Minutes
Application #AN3080
Conditions Analytes
1. Uroporphyrin I
(R1:A, R2:P, R3:A, R4:P, R5:A, R6:P, R7:A, R8:P)
(m/z 831 727, 623, 655)
2. 7-Carboxyporphyrin I
(R1:A, R2:P, R3:A, R4:P, R5:A, R6:P, R7:M, R8:P)
(m/z 787 683, 670, 623)
3. 6-Carboxyporphyrin I
(R1:M, R2:P, R3:A, R4:P, R5:A, R6:P, R7:M, R8:P)
(m/z 743 639, 507, 521)
4. 5-Carboxyporphyrin I
(R1:M, R2:P, R3:M, R4:P, R5:A, R6:P, R7:M, R8:P)
(m/z 699 463, 595, 640)
5. Coproporphyrin I
(R1:M, R2:P, R3:M, R4:P, R5:M, R6:P, R7:M, R8:P)
(m/z 655 537, 596, 523)
6. Coproporphyrin III
(R1:M, R2:P, R3:M, R4:P, R5:M, R6:P, R7:P, R8:M)
(m/z 655 537, 596, 523)
7. Mesoporphyrin IX
(R1:M, R2:E, R3:M, R4:E, R5:M, R6:P, R7:M, R8:P)
(m/z 567 449, 479, 508)
8. Protoporphyrin IX
(R1:M, R2:V, R3:M, R4:V, R5:M, R6:P, R7:P, R8:M)
(m/z 563 445, 504, 489)
Where:
A: -CH2COOH
E: -CH2CH3
M: -CH3
P: -CH2CH2COOH
V: -CH=CH2
Relative Intensity