ACE UHPLC and HPLC Columns
Analytes
1. 3-Aminophthalic acid
2. Hydrolysis product of pomalidomide
3. Hydrolysis product of pomalidomide
4. Glucuronide conjugate of 5-hydroxy pomalidomide
5. Glucuronide conjugate of 5-hydroxy pomalidomide
6. 7-Hydroxy pomalidomide
7. 5-Hydroxy pomalidomide
8. Pomalidomide
9. N-Acetyl pomalidomide
4
5
5
Time - Minutes
Application #AN4240
Plasma (2.5h pooled sample)
3
3
1 2
4
6
6
7
Urine (0-72h pooled sample)
7
8
8 9
Pomalidomide
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Polyethylene Glycol 1000
Column: ACE 3 C8
Dimensions: 150 x 4.6 mm
Part Number: ACE-112-1546
Mobile Phase: A: 0.1% formic acid in H2O
B: MeOH
Gradient: Time (mins) %B
0 50
45 85
50 50
60 50
Flow Rate: 1 mL/min
Detection: APCI (negative ion)
Application #AN3900
Conditions
1.00e8
8.00e7
6.00e7
4.00e7
2.00e7
0.0
Intensity (cps)
Reproduced with permission of Wickham Laboratories, UK
2 4 6 8 10 12 14 16 18 20 22 24 26 28
1.20e8
Time - Minutes
Po14CPomalidomide and Metabolites in Human Plasma and Urine
Conditions
Column: ACE 3 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-111-1546
Mobile Phase: A: 25 mM ammonium
acetate pH 5.5 in H2O
B: MeOH
Gradient: Time (mins) %B
0 0
2 0
38 36
44 100
48 100
50 0
Flow Rate: 0.7 mL/min
Temperature: 30 °C
Detection: Radiometric
Metabolites characterised using
LC-MS/MS (positive ion mode)
cpm cpm
Hoffmann M, Kasserra C, Reyes J, Schafer P, Kosek J, Capone L, Parton A, Kim-Kang H, Surapaneni S, Kumar G. Absorption, Metabolism and Excretion of 14C
Pomalidomide in Humans following Oral Administration. Cancer Chemotherapy and Pharmacology 71, 489-501 (2013) doi 10.1007/s00280-012-2040-6