ACE UHPLC and HPLC Columns
Pharmaceutically Relevant Compounds (V)
Conditions
Column: ACE 3 C18-PFP
Dimensions: 50 x 2.1 mm
Part Number: ACE-1110-0502
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeOH
Gradient: Time (mins) %B
0 3
5 100
Flow Rate: 0.6 mL/min
Temperature: 40 °C
Detection: UV, 254 nm
Application #AN2500
Analytes
1. Sulphanilamide
2. Nizatidine
3. Metronidazole
4. Amiloride
5. Hydrochlorothiazide
6. Caffeine
7. Pindolol
8. Metoprolol
9. Phenacetin
10. 1,3-Dinitrobenzene
11. Hexobarbital
12. Furosemide
13. Piroxicam
14. Carvedilol
15. Ketoprofen
16. Ibuprofen
17. Indomethacin
2
14
0 1 2 3 4 min
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Pharmaceutically Relevant Mixture (I) – Different Selectivity Using pH
Conditions
134
9
1
3
2
4
5
7
6
8
11
10
12
14
13
15 16
17
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1
0 1
2
3
3
4
4
5
6
7
8
9
10
12
13
15 16
17
11
Time - Minutes
Column: ACE Ultracore 2.5 SuperC18
Dimensions: 50 x 2.1 mm
Part Number: CORE-25A-0502U
Mobile Phase: A1: 10 mM ammonium formate pH 3 in H2O
A2: 0.1% ammonia pH 10.7 in H2O
B1: 10 mM ammonium formate pH 3
in MeCN/H2O (90:10 v/v)
B2: 0.1% ammonia pH 10.7 in
MeCN/H2O (90:10 v/v)
Gradient: Time (mins) %B
0 3
5 100
6 100
Flow Rate: 0.6 mL/min
Temperature: 40 °C
Detection: UV, 254 nm
Application #AN1310
Analytes
1. Atenolol
2. Methylphenylsulfoxide
3. Eserine
4. Prilocaine
5. Bupivacaine
6. Tetracaine
7. 1,2,3,4-Tetrahydro-1-Naphthol
8. Carvedilol
9. Nitrobenzene
10. Methdilazine
11. Amitriptyline
12. Valerophenone 4
10
2 12
2 12
5
3
10
11
6
7
1
8, 9
4
4
5
5
3
3
11
11
6
6
7
7
2
2
12
12
1
1
8
8
9
9
10
10
pH 3
1 2 3 4 5
min
pH 3
ppHH 1100..77
1
2
3
4
7
9
8
6
12
5
10
11
4
5
3
11
6
7
1
8, 9
1 2 3 4 5
min
pH 3
pH 10.7
1
2
3
4
5
6
8,9
10
11
12
Time - Minutes