ACE UHPLC and HPLC Columns
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 2.1 mm
Part Number: CORE-25A-1002U
Mobile Phase: A: 5 mM ammonium formate in H2O/MeOH (9:1 v/v)
B: 5 mM ammonium formate in H2O/MeOH (1:9 v/v)
Gradient: Time (mins) %B
0.0 30
0.5 30
15.0 100
22.0 100
22.1 30
27.0 30
Flow Rate: 0.3 mL/min
Injection: 6 μL
Temperature: 24 °C
Detection: AB SCIEX 4000 QTRAP
TurbolonSpray ESI positive mode
Capillary voltage: 5000 V
Heater gas temperature: 450 °C
Sample: Sample prepared using QuEChERs methodology
Method validated using cucumber matrix spiked at
0.01 mg/kg. 265 analytes successfully validated
(Analytes in black)
Time - Minutes
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 127
Pes300 Pesticide Screen by LC-MS/MS
Application #AN3120
Conditions
Intensity, cps
Reproduced with permission of National Food Chain Safety Office, Directorate of Plant Protection, Soil Conservation and Agri-Environment, Hungary
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