ACE UHPLC and HPLC Columns
Time - Minutes
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Pes250 Pesticide Screen by LC-MS/MS
Column: ACE Excel 2 C18
Dimensions: 100 x 2.1 mm
Part Number: EXL -101-1002U
Mobile Phase: A: 10 mM ammonium formate + 0.05%
formic acid in H2O
B: 10 mM ammonium formate + 0.05%
formic acid in MeOH
Gradient: Time (mins) %B
0.00 2
0.25 30
10.00 100
12.00 100
12.50 2
14.50 2
Flow Rate: 0.5 mL/min
Temperature: 50 °C
Detection: TSQ Quantiva triple quad MS
Positive mode HESI
Spray voltage: 3500 V
Ion transfer tube temperature: 350 °C
Vaporizer temperature: 300 °C
Application #AN3060
Conditions
1.6E6
1.5E6
1.4E6
1.3E6
1.2E6
1.1E6
1.0E6
9.0E5
8.0E5
7.0E5
6.0E5
5.0E5
4.0E5
3.0E5
2.0E5
1.0E5
0
0 1 2 3 4 5 6 7 8 9 10
Time (min)
Reproduced with permission of Centre for Analytical Chemistry, California Department of Food and Agriculture, USA
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