ACE UHPLC and HPLC Columns
1. Heptafluorobutyric acid
(m/z 212.9 168.9)
2. Perfluorohexanoic acid
(m/z 313 268.9)
3. Perfluorobutanesulfonic acid
(m/z 299 79.9)
Intensity, cps
Perfluoroalkyl Substances by Ion-Pairing LC-MS/MS
7
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Perfluoro Acids by LC-MS/MS
Column: ACE Excel 2 C18
Dimensions: 50 x 2.1 mm
Part Number: EXL -101-0502U
Mobile Phase: A: 2 mM ammonium acetate,
0.1% acetic acid/MeCN (95:5 v/v)
B: 2 mM ammonium acetate,
0.1% acetic acid/MeCN (5:95 v/v)
Gradient: Time (mins) %B
0.0 25
0.5 25
5.5 95
7.5 95
8.0 25
10.0 25
Flow Rate: 0.5 mL/min
Injection: 20 μL
Temperature: 40 °C
Detection: AB SCIEX triple quad 5500
Negative ESI MRM
Source temperature: 450 °C
IonSpray voltage: -2400 V
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 50 x 2.1 mm
Part Number: CORE-25A-0502U
Mobile Phase: A: 2 mM ammonium acetate + 5 mM
1-methylpiperidine in MeOH/H2O (5:95 v/v)
B: 2 mM ammonium acetate + 5 mM
1-methylpiperidine in MeOH/H2O (95:5 v/v)
Gradient: Time (mins) %B
0.0 10
0.3 10
1.0 20
1.5 50
5.0 80
10.0 80
13.0 100
16.0 100
Flow Rate: 0.3 mL/min
Injection: 5 μL
Temperature: 35 °C
Detection: Agilent 6430 triple quad MS
ESI in negative ion mode
Capillary voltage: 3000 V
Nebulizer pressure: 50 psi
Application #AN1280
Application #AN2560
Conditions
Conditions
Analytes
Analytes
Reproduced with permission of Biotage GB Ltd, UK
4. Perfluoroheptanoic acid
(m/z 363 319)
5. Perfluorooctanoic acid
(m/z 413 368.9)
6. Perfluorohexanesulfonic acid
(m/z 399 80)
7. Perfluorononanoic acid
(m/z 463 419)
8. Perfluorodecanoic acid
(m/z 513 469)
9. Perfluorooctanesulfonic acid
(m/z 499 80)
1
1
0 1
18000
16000
14000
12000
10000
8000
6000
4000
2000
0
2
2
2
4
4
4
5
5
5
6
6
6
7
7
8
8
8
9
9
9
10
10
11
3
3
3
Time - Minutes
1. PFBA
(m/z 213 169)
2. PFPeA
(m/z 263 219)
(m/z 263 175)
3. PFBS
(m/z 299 99)
(m/z 299 80)
4. PFHxA
(m/z 313 269)
(m/z 313 119)
5. PFHpA
(m/z 363 319)
(m/z 363 169)
6. PFHxS
(m/z 399 99)
(m/z 399 80)
7. PFOA
(m/z 413 369)
(m/z 413 169)
8. PFNA
(m/z 463 419)
(m/z 463 169)
9. PFOS
(m/z 499 99)
(m/z 499 80)
10. FOSA
(m/z 498 498)
(m/z 498 78)
11. PFDA
(m/z 513 469)
(m/z 513 269)
Time - Minutes
Reproduced with permission of Department of Analytical Chemistry, Faculty of Science and Technology, University of the Basque Country, Spain