ACE UHPLC and HPLC Columns
Peptides – Selectivity Changes with Bonded Phase and Mobile Phase
Column: ACE 5 C18-300; ACE 5 C8-300; ACE 5 C4-300;
ACE 5 Phenyl-300; ACE 5 CN-300
Dimensions: 250 x 4.6 mm
Part Number: ACE-221-2546, ACE-222-2546, ACE-223-2546,
ACE-225-2546, ACE-224-2546
Mobile Phase: A: 0.1% TFA or 0.1% formic acid in H2O
B: MeCN
Gradient: Time (mins) %B
0.0 10
25.0 40
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 220 nm
120
0.1% TFA 0.1% HCOOH
5
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Application #AN3430
Conditions Analytes
1. Gly-Tyr (MW: 238.34)
2. Val-Tyr-Val (MW: 379.45)
3. Methionine enkephalin (MW: 573.67)
4. Angiotensin II (MW: 1046.18)
5. Leucine enkephalin (MW: 555.62)
ACE 5 C18-300
ACE 5 C8-300
ACE 5 C4-300
ACE 5 Phenyl-300
ACE 5 CN-300
ACE 5 C18-300
ACE 5 C8-300
ACE 5 C4-300
ACE 5 Phenyl-300
ACE 5 CN-300
1
2 3 4 5
1
2
3 4 5
1
2
3
4,5
1
2
3
4
5
1
2
3 4
1
2
3,4
5
1
2 3 4 5
1
2 3 4 5
1
2 4 3 5
1
2
3
4 5
Time - Minutes Time - Minutes
