ACE UHPLC and HPLC Columns
Peptide Test Mix
Conditions Analytes
Column: ACE 5 C18-300
Dimensions: 250 x 4.6 mm
Part Number: ACE-221-2546
Mobile Phase: A: 0.1% TFA in H2O
B: 0.1% TFA in MeCN
Gradient: Time (mins) %B
0 10
25 40
Flow Rate: 2 mL/min
Injection: 5 μL
Temperature: Ambient
Detection: UV, 220 nm
Application #AN3930
1. Gly-Tyr (MW: 238.34)
2. Oxytocin (MW: 1007)
3. Angiotensin II (MW: 1046.18)
4. Neurotensin (MW: 1672.92)
Sesamol Desfluoroparoxetine Paroxetine
1
2
3
0 1 2 3 4 5 6 7
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Paroxetine and Desfluoro Analogue
Column: ACE 5 CN
Dimensions: 150 x 4.6 mm
Part Number: ACE-124-1546
Mobile Phase: 20 mM ammonium formate
pH 3.0/MeOH (60:40 v/v)
Flow Rate: 2 mL/min
Injection: 20 μL
Temperature: Ambient
Detection: UV, 295 nm
Application #AN3890
Conditions Analytes
1. Sesamol
2. Desfluoroparoxetine
3. Paroxetine
Reproduced with permission of School of Pharmacy, University of Sunderland, UK
1
2
3
4
Time - Minutes Time - Minutes
Peptides – Varying pH
Column: ACE 5 C18-300
Dimensions: 250 x 4.6 mm
Part Number: ACE-221-2546
Mobile Phase: A: 20 mM KH2PO4 in
H2O (pH as indicated)
B: MeCN
Gradient: Time (mins) %B
0 10
25 40
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 220 nm
Application #AN3990
Conditions Analytes
1. Gly-Tyr
(MW: 238.34)
2. Val-Tyr-Val
(MW: 379.45)
3. Methionine enkephalin
(MW: 573.67)
4. Angiotensin II
(MW: 1046.18)
5. Leucine enkephalin
(MW: 555.62)
1
2
3 4 5
1
2
3 4 5
1
2
3 4 5
Time - Minutes
pH 2.0
pH 4.4
pH 6.5
Response - MilliVolts
Response - MilliVolts