ACE UHPLC and HPLC Columns
Paracetamol and Related Substances – Enhanced Resolution
100
80
60
40
20
Paracetamol and Related Substances – Phase Selectivity
ACE Excel 2 C18-PFP 100 x 3.0 mm
ACE Excel 2 C18-Amide 100 x 3.0 mm
ACE Excel 2 CN-ES 100 x 3.0 mm
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Column: ACE Excel 2 C18-PFP,
ACE Excel 2 C18-Amide,
ACE Excel 2 CN-ES
Dimensions: 100 x 3.0 mm
Part Number: EXL -1010-1003U,
EXL -1012-1003U,
EXL -1013-1003U
Mobile Phase: A: 20 mM ammonium acetate
pH 6.0 in H2O
B: 20 mM ammonium acetate
pH 6.0 in MeOH/H2O (90:10 v/v)
Gradient: Time (mins) %B
0.0 5
10.0 95
12.5 95
13.0 5
Flow Rate: 1.2 mL/min
Injection: 2 μL
Temperature: 40 °C
Detection: UV, 210 nm
Application #AN3580
Conditions Analytes
1. 4-Aminophenol
2. Hydroquinone
3. 2-Aminophenol
4. Paracetamol
5. 2-Acetamidophenol
6. Phenol
7. 4-Nitrophenol
8. 2-Nitrophenol
9. 4-Chloroacetanilide
10. 4-Chlorophenol
ACE Excel 2 C18-PFP 100 x 3.0 mm
ACE Excel 2 C18-Amide 100 x 3.0 mm
ACE Excel 2 CN-ES 100 x 3.0 mm
1
2
3 4
5
7
8
910
1
2
3 4
5
6
7
8
9
10
1
2
4 3
6
7
8
9
10
2
4
6
8
min
1
3
4
6
9 10
1
2
3
4
5
6
10
1
2
4 3
5
10
Time - Minutes
Column: ACE Excel 1.7 C18
Dimensions: 100 x 3.0 mm
Part Number: EXL -171-1003U
Mobile Phase: A: 10 mM ammonium acetate
pH 6.0 in H2O
B: 10 mM ammonium acetate
pH 6.0 in MeOH/H2O (90:10 v/v)
Gradient: Time (mins) %B
0.00 5
0.21 5
10.23 95
13.56 95
14.16 5
20.24 5
Flow Rate: 0.51 mL/min
Injection: 1.4 μL
Temperature: 40 °C
Detection: UV, 230 nm
Application #AN2220
Conditions Analytes
1. 4-Aminophenol
2. Hydroquinone
3. Paracetamol
4. 2-Acetamidophenol
5. Phenol
6. 4-Nitrophenol
7. 2-Nitrophenol
8. 4-Chloroacetanilide
0 2.5 5 7.5 10 12.5
mAU
0
1
2
3
4
5
7
6
8
0 5 10 12.5 min
For enhanced speed of paracetamol and related compounds, see AN2210.
Time - Minutes
2
5
4
3
6
7
8
1