ACE UHPLC and HPLC Columns
Paraben Preservatives
i-Butyl paraben n-Butyl paraben
Benzyl paraben
Paracetamol and Related Compounds
116
Methyl paraben Ethyl paraben n-Propyl paraben
1
2
0 2 4 6 8 10 12 14 16 18 Time min
Intens.
mAU
-10
-15
-20
-25
-30
1
2
3
4
5
6
8
9
10
Paracetamol and Related Substances
– Fast Analysis (I) Application #AN2210
Conditions Analytes
100
80
60
40
20
2
4 6
5
7
3
1
Time - Minutes Time - Minutes
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3
4 5
6
2 4 6 8 10 12 14 16 18 mins
1 2 3 4 5 6 7
min
0
4 6
mAU
0
1
2
3
4
5
7
6
8
8
0 2 4 6 min
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 3.0 mm
Part Number: EXL -1010-1003U
Mobile Phase: A: 20 mM ammonium
acetate pH 6.0 in H2O
B: 20 mM ammonium acetate
pH 6.0 in MeOH/H2O (90:10 v/v)
Gradient: Time (mins) %B
0.0 6
5.5 63
Flow Rate: 1.2 mL/min
Injection: 2 μL
Temperature: 27 °C
Detection: UV, 220 nm
Application #AN1260
Conditions Analytes
1. 4-Aminophenol
2. Hydroquinone
3. 2-Aminophenol
4. Paracetamol
5. 2-Acetamidophenol
6. Phenol
7. 4-Nitrophenol
8. 2-Nitrophenol
9. 4-Chloroacetanilide
10. 4-Chlorophenol
1
1
2
3
4
5
7
5
6
9
10
Column: ACE Excel 1.7 C18
Dimensions: 50 x 3.0 mm
Part Number: EXL -171-0503U
Mobile Phase: A: 10 mM ammonium
acetate pH 6.0 in H2O
B: 10 mM ammonium
acetate pH 6.0 in
MeOH/H2O (90:10 v/v)
Gradient: Time (mins) %B
0.00 5
0.08 5
5.08 95
6.76 95
7.09 5
10.00 5
Flow Rate: 0.51 mL/min
Injection: 0.7 μL
Temperature: 40 °C
Detection: UV, 230 nm
1. 4-Aminophenol
2. Hydroquinone
3. Paracetamol
4. 2-Acetamidophenol
5. Phenol
6. 4-Nitrophenol
7. 2-Nitrophenol
8. 4-Chloroacetanilide
For enhanced resolution of paracetamol and related compounds, see AN2220.
Column: ACE 3 Phenyl
Dimensions: 150 x 2.1 mm
Part Number: ACE-115-1502
Mobile Phase: 25 mM ammonium
acetate pH 6.8 in
H2O/MeOH (50:50 v/v)
Flow Rate: 0.2 mL/min
Injection: 2 μL
Temperature: 40 °C
Detection: UV, 240 nm
Application #AN1250
Conditions Analytes
1. Methyl paraben
2. Ethyl paraben
3. n-Propyl paraben
4. i-Butyl paraben
5. n-Butyl paraben
6. Benzyl paraben
Reproduced with permission of The Chemical Analysis Facility, University of Reading
Time - Minutes