ACE UHPLC and HPLC Columns
Oxymetazoline in Nasal Spray Formulation
Application #AN3660
Oxymetazoline
Benzalkonium chloride
Paclitaxel
Response -
MilliVolts
1600
1400
1200
1000
800
600
400
200
Paeonia Lactiflora Extract HPLC Fingerprint
Application #AN3820
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Conditions Analytes
1
2
Column: ACE 5 CN
Dimensions: 150 x 4.6 mm
Part Number: ACE-124-1546
Mobile Phase: aq. Na2HPO4 pH 7.0/MeCN
(50:50 v/v)
Flow Rate: 1.5 mL/min
Temperature: 30 °C
Detection: UV, 214 nm
1. Oxymetazoline
2. Benzalkonium chloride
0 2 4 6 8 10 12 14 16
24
22
20
18
16
14
12
10
8
6
4
2
0
Response - MilliVolts
Time - Minutes
Reproduced with permission of Thornton & Ross Ltd, Huddersfield, UK
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: H2O/MeCN (45:55 v/v)
Flow Rate: 1 mL/min
Temperature: 40 °C
Detection: UV, 227 nm
Application #AN3670
Conditions Analyte
1. Paclitaxel
Paclitaxel
1
Time - Minutes
Reproduced with permission of Biointeractions Ltd, Reading, UK
Conditions Analytes
Column: ACE 5 C18
1. Gallic acid
Dimensions: 250 x 4.6 mm
2. Catechin hydrate
Part Number: ACE-121-2546
3. Methyl gallate
Mobile Phase: A: 0.1% phosphoric acid
4. Paeoniflorin
in HO
5. Benzoic acid
2 B: MeCN
Gradient: Time (mins) %B
0 10
5 15
25 22
45 70
46 80
50 80
Flow Rate: 1 mL/min
Injection: 20 μL
Temperature: 25 °C
Detection: UV, 254 nm
Sample: P. lactiflora root extracted with boiling water and
polysaccharides removed by precipitation
Paeoniflorin
1
2
0 10
0
20 30 40 50
3
4
5
Time - Minutes
Choi H-J, Chung T-W, Park M-J, Lee KS, Yoon Y, Kim HS, Lee JH, Kwon S-M, Lee S-O, Kim K-J, Baek J-H, Ha K-T. (2016) Paeonia lactiflora Enhances the Adhesion
of Trophoblast to the Endometrium via Induction of Leukemia Inhibitory Factor Expression. PloS ONE 11(2): e0148232. doi:10.137/journal.pone.0148232